Squash leaf curl virus (SLCV): a serious disease threatening cucurbits production in Palestine
Squash leaf curl virus (SLCV): a serious disease threatening cucurbits production in Palestine
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DOI：
10.1007/s11262-013-1012-1
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发表时间：
2014-04-01
期刊：
影响因子：
1.6
通讯作者：
Abu-Zeitoun, S. Y.
中科院分区：
文献类型：
--
作者：
Ali-Shtayeh, M. S.;Jamous, R. M.;Abu-Zeitoun, S. Y.
本研究描述了西葫芦曲叶病的发病情况和西葫芦曲叶病毒巴勒斯坦分离物[SLCV-（PAL）]的分子特征。从南瓜（西葫芦）、西瓜[西瓜（Citrullus lanatus（Thunb.）]，和黄瓜（Cucumis sativus L.）通过PCR和RCA测试植物的SLCV-[PAL]感染。SLCV也被发现自然发生在藜，旋花，和Prosporis farcta，显示变黄。夏季采集自纳布卢斯的样品发病率为85%，秋季采集自卡勒基利亚的样品发病率为98%。另一方面，SLCV在冬季的发病率不超过25%.对SLCV-[PAL]的DNA-A和DNA-B基因组进行了扩增和序列测定，并将其序列存入GenBank。序列分析表明，SLCV-[PAL]与来自黎巴嫩（SLCV-LB 2）、约旦（SLCV-JO）、以色列（SLCV-IL）和埃及（SLCV-EG）的其他分离物密切相关。SLCV-[PAL]的DNA-A与SLCV-JO和SLCV-LB 2的核苷酸同源性最高（99.4%），而DNA-B与SLCV-IL的核苷酸同源性最高（99.3%）。然而，在基因组测序后，发现由于两个单独的点突变，在一些SLCV巴勒斯坦分离株中两个病毒开放阅读框（ORF）被改变。AC 2 ORF延长了141个核苷酸，而AC 4 ORF延长了36个核苷酸。
The incidence of squash leaf curl disease and molecular characterization of the Palestinian isolate of Squash leaf curl virus [SLCV-(PAL)] are described in this study. Symptomatic leaf samples obtained from squash (Cucurbita pepo), watermelon [Citrullus lanatus (Thunb.)], and cucumber (Cucumis sativus L.) plants were tested for SLCV-[PAL] infection by PCR and RCA. SLCV was also found to occur naturally in Chenopodium murale, Convolvulus sp, and Prosporis farcta which showed yellowing. The disease incidence was 85 % in samples collected from Nablus in summer season, while it was 98 % in samples collected from Qalqilia in autumn. On the other hand, SLCV incidence did not exceed 25 % in winter season. The full-length DNA-A and DNA-B genomes of SLCV-[PAL] were amplified and sequenced, and the sequences were deposited in the GenBank. Sequence analysis reveals that SLCV-[PAL] is closely related to other isolates from Lebanon (SLCV-LB2), Jordan (SLCV-JO), Israel (SLCV-IL), and Egypt (SLCV-EG). DNA-A of SLCV-[PAL] showed the highest nucleotide identity (99.4 %) with SLCV-JO, and SLCV-LB2, while DNA-B had the highest nucleotide identity (99.3 %) with SLCV-IL. However, following genome sequencing, it was found that due to two separate point mutations, two viral open reading frames (ORF) were altered in some SLCV Palestinian isolates. The AC2 ORF was extended by 141 nucleotides, while the AC4 ORF was extended by 36 nucleotides.