Acoustic methods for high-throughput protein crystal mounting at next-generation macromolecular crystallographic beamlines
Acoustic methods for high-throughput protein crystal mounting at next-generation macromolecular crystallographic beamlines
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DOI：
10.1107/s0909049513020372
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发表时间：
2013-09-01
影响因子：
2.5
通讯作者：
Heroux, Annie
中科院分区：
文献类型：
--
作者：
Roessler, Christian G.;Kuczewski, Anthony;Heroux, Annie
为了充分利用先进的数据收集技术和下一代大分子晶体光束线的高束通量，需要快速可靠的方法来每秒安装和对准许多样品。一种方法是使用声波喷射器将含有晶体的液滴喷射到固体X射线透明表面上，然后可以定位和旋转以收集数据。概念验证实验是在国家同步加速器光源上对声学地喷射到聚酰亚胺传送带上的热裂解晶体进行的。在每个晶体上收集了小楔形数据，并从这些晶体的衍射性很好的子集组装成完整的数据集。未来的发展和实施将侧重于实现以每秒100多个液滴的速度喷射和平移单个液滴。
To take full advantage of advanced data collection techniques and high beam flux at next-generation macromolecular crystallography beamlines, rapid and reliable methods will be needed to mount and align many samples per second. One approach is to use an acoustic ejector to eject crystal-containing droplets onto a solid X-ray transparent surface, which can then be positioned and rotated for data collection. Proof-of-concept experiments were conducted at the National Synchrotron Light Source on thermolysin crystals acoustically ejected onto a polyimide 'conveyor belt'. Small wedges of data were collected on each crystal, and a complete dataset was assembled from a well diffracting subset of these crystals. Future developments and implementation will focus on achieving ejection and translation of single droplets at a rate of over one hundred per second.