Intravitreal injection of hyaluronidase cannot induce posterior vitreous detachment in the rabbit
Intravitreal injection of hyaluronidase cannot induce posterior vitreous detachment in the rabbit
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DOI：
10.1097/00006982-200003000-00013
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发表时间：
2000-01-01
影响因子：
3.3
通讯作者：
Yoshida, A
中科院分区：
文献类型：
--
作者：
Hikichi, T;Kado, M;Yoshida, A
目的：探讨玻璃体腔内注射透明质酸酶能否诱导兔玻璃体后脱离。方法：12只新西兰大白兔各1只眼经玻璃体平坦部玻璃体腔内注射透明质酸酶20IU(0.1mT在无菌平衡盐溶液中配制)。对侧眼玻璃体内注射BSS 0.1mT，分别于注射后3个月和6个月处死4只和8只兔，摘除眼球。结果：在注射后3个月和6个月时，扫描电子显微镜显示，注射透明质酸酶或BSS组的视网膜表面覆盖有玻璃体胶原纤维。没有一只眼，即使是接受透明质酸酶治疗6个月以上的眼，其光滑的视网膜表面与裸露的内部限制层一致，这表明PVD的发生。结论：透明质酸酶不能在玻璃体注射后的g个月内诱发兔PVD。
Purpose: To investigate whether intravitreal injection of hyaluronidase can induce posterior vitreous detachment (PVD) in the rabbit.Methods: One eye each of 12 New Zealand white rabbits received intravitreal injection via the pars plana of 20 IU of hyaluronidase (0.1 mt reconstituted in sterile balanced salt solution [BSS]) into the midvitreous cavity. The fellow eye of each rabbit received a Vitreous injection of 0.1 mt of BSS, At 3 and 6 months after intravitreal injection, four and eight rabbits were killed, respectively, and the eyes were enucleated. After fixation, scanning electron microscopy was performed to study the vitreoretinal interface.Results: At 3 and 6 months after injection, scanning electron microscopy showed that the retinal surfaces in eyes that received either hyaluronidase or BSS were covered with vitreous collagen fibers. No eyes, even those that received hyaluronidase over a period of 6 months, had the smooth retinal surface consistent with a bare internal limiting lamina that suggests the development of PVD.Conclusion: Hyaluronidase cannot induce PVD in the rabbit over a g-month period after vitreous injection.