Map-based cloning of qBWT-c12 discovered brassinosteroid-mediated control of organ size in cotton.
Map-based cloning of qBWT-c12 discovered brassinosteroid-mediated control of organ size in cotton.
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DOI：
10.1016/j.plantsci.2019.110315
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发表时间：
2020-02
期刊：
影响因子：
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通讯作者：
M. M. Ahmed-M.;Cong Huang;Chao Shen;A. Khan;Zhongxu Lin
中科院分区：
文献类型：
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作者：
M. M. Ahmed-M.;Cong Huang;Chao Shen;A. Khan;Zhongxu Lin
确保纤维产量稳定是棉花育种者的首要目标，因为世界人口在增加，对棉纤维的需求每年都在增加。因此，提高棉铃平均重可以提高籽棉产量，最终达到提高棉纤维产量的目的。本研究完成了棉铃重调节基因qBWT-c12的定位克隆。散装分离分析检测到连锁标记，帮助检测到一个稳定的BWT调节位点qBWT-c12，在一个新的铃大小突变体BS41的Chr12上。在重组衍生的f3和f4群体中，qbwt -c12在AD-A12_07和AD-FM_44标记之间的间隔为0.89 cM。同源定位在AD-FM_44克隆序列中检测到一个40 bp的插入-删除（InDel）位点，位于ghbrh1_a12下游+341处，与BWT表型完全连锁。ghbrh1_a12的抑制表达表明其参与了BS41的早期铃发育事件。油菜素内酯（brassinosteroids， BR）生物合成和信号通路基因在不同组织中表达上调，但器官生长受到抑制，导致植株矮化、叶片变小、棉铃变形变小。因此，预计BR信号级联的中断可能与BS41中ghbrh1_a12的低表达有关。本研究首次报道了棉铃大小调控的遗传解剖。取得。利用基因图谱克隆BWT调控位点qBWT-c12。此外，还强调了ghbrh1_a12在调节BR稳态中的作用及其调节棉花植株生长和棉铃发育的潜力。
Assuring fiber yield stability is the primary objective for cotton breeders since the world population is on the rise, and the demand for cotton fiber is increasing every year. Thus, enhancing average cotton boll weight (BWT) could improve seed cotton production, and ultimately to increase cotton fiber yield. This study accomplished the map-based cloning of a novel boll weight regulating locus,qBWT-c12, in cotton. Bulk segregation analysis detected linked markers, aided in the detection of a stable BWT regulating locus,qBWT-c12, on Chr12 in a novel boll size mutant, BS41. Progeny evaluation confined theqBWT-c12to a 0.89 cM interval between the AD-A12_07 and AD-FM_44 markers in recombinant derived F3and F4populations. Homology mapping detected a 40 bp insertion-deletion (InDel) site in the AD-FM_44 clone sequence situated +341 downstream ofGhBRH1_A12, which showed complete linkage to the BWT phenotype. The suppressed expression ofGhBRH1_A12suggested its putative involvement during early boll development events in BS41. Although brassinosteroid (BR) biosynthesis and signaling pathway genes were up regulated in different tissues, but the organ growth was suppressed leading to dwarf plants, smaller leaves, and de-morphed smaller bolls in BS41. Thus, a disruption in the BR signal cascade is anticipated and could be related to lowerGhBRH1_A12expression in BS41.This study firstly reported the genetic dissection of boll size regulation ofG. barbadenseinG.hirsutumbackground using map-based cloning of a BWT regulating locus,qBWT-c12. Moreover, it also emphasized the putative roleGhBRH1_A12in regulating BR homeostasis and its potential to modulate plant growth and boll development in cotton.