The recovery of ligno cellulose-degrading enzymes from spent mushroom compost
The recovery of ligno cellulose-degrading enzymes from spent mushroom compost
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DOI：
10.1016/0960-8524(95)00153-0
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发表时间：
1995-01-01
影响因子：
11.4
通讯作者：
Jackson, AM
中科院分区：
文献类型：
--
作者：
Ball, AS;Jackson, AM
利用各种物理和化学处理方法对废弃蘑菇堆肥中木质纤维素降解酶的回收进行了评估。经过混合物理处理的废旧堆肥提取液中木聚糖酶活性的最大回收率最高。混合堆肥提取物中也恢复了一系列木质纤维素的降解活性；过氧化物酶、木聚糖脱支酶乙酰酯酶和阿拉伯呋喃糖苷酶，以及纤维素降解活性内切葡聚糖酶、纤维二糖水合酶和β-葡萄糖苷酶。堆肥提取物还能从麦草中释放还原糖(12nmolmin(-1)g堆肥(-1))。对该酶活性稳定性的研究表明，在65℃下培养1h，pH为7~9时酶活性最高。该酶的活性和稳定性表明，从堆肥提取液中提取的酶鸡尾酒在草类木质纤维素生物转化为动物饲料方面具有潜在的作用。
The recovery of lignocellulose-degrading enzymes from spent mushroom compost was assessed using a variety of physical and chemical treatments. Maximum recoveries of active xylanase activity were detected in extracts from spent compost which had been physically treated by blending. A range of lignocellulose-degrading activities was also recovered from blended compost extracts; peroxidases, the xylan-debranching enzymes acetylesterase and arabinofuranosidase, and the cellolose-degrading activities endoglucanase, cellobiohydrase and beta-glucosidase. The compost extract was also shown to be capable of releasing reducing sugars (12 nmol min(-1) g compost(-1)) from wheat straw. Characterization of the stability of this enzyme activity showed that maximum activity could be detected between pH 7 and 9 and after 1 h incubation at 65 degrees C. The activity and stability of the enzymes suggest a potential role for enzyme cocktails from compost extracts in the biological upgrading of grass lignocelluloses to animal fodder.