The Condensed Protocols From Molecular Cloning: A Laboratory Manual
The Condensed Protocols From Molecular Cloning: A Laboratory Manual
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发表时间：
2006
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通讯作者：
David W. Russell;J. Sambrook
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文献类型：
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作者：
David W. Russell;J. Sambrook
1.在分子克隆中使用质粒载体2.噬菌体bb及其载体3.使用噬菌体M13载体4.使用高容量向量5. DNA的凝胶电泳和脉冲场琼脂糖凝胶电泳6.真核基因组DNA的制备与分析7.从真核细胞中提取、纯化和分析mRNA 8.通过聚合酶链反应体外扩增DNA 9.放射性标记的DNA和RNA探针的制备11.我的超次元帝国cDNA文库的制备和基因鉴定12. 13.第十三章14. honeymoon筛选表达文库15.克隆基因在大肠杆菌16中的表达将克隆的基因引入培养的哺乳动物细胞17.哺乳动物细胞中的基因表达分析18.蛋白质相互作用技术
1. Using Plasmid Vectors in Molecular Cloning 2. Bacteriophage bb and Its Vectors 3. Working with Bacteriophage M13 Vectors 4. Working with High-capacity Vectors 5. Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis 6. Preparation and Analysis of Eukaryotic Genomic DNA 7. Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells 8. In Vitro Amplification of DNA by the Polymerase Chain Reaction 9. Preparation of Radiolabeled DNA and RNA Probes 10. Working with Synthetic Oligonucleotide Probes 11. Preparation of cDNA Libraries and Gene Identification 12. DNA Sequencing 13. Mutagenesis 14. Screening Expression Libraries 15. Expression of Cloned Genes in Escherichia coli 16. Introducing Cloned Genes into Cultured Mammalian Cells 17. Analysis of Gene Expression in Mammalian Cells 18. Protein Interaction Technologies Appendices Index