Methermicoccus shengliensis gen. nov., sp nov., a thermophilic, methylotrophic methanogen isolated from oil-production water, and proposal of Methermicoccaceae fam. nov.
Methermicoccus shengliensis gen. nov., sp nov., a thermophilic, methylotrophic methanogen isolated from oil-production water, and proposal of Methermicoccaceae fam. nov.
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DOI：
10.1099/ijs.0.65049-0
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发表时间：
2007-12-01
影响因子：
2.8
通讯作者：
Zhang, Hui
中科院分区：
文献类型：
--
作者：
Cheng, Lei;Qiu, Tian-Lei;Zhang, Hui
从中国的Shengli油田中分离出一种嗜热的甲基营养甲烷菌株ZC-1（T）。 ZC-1（T）菌株的细胞是运动球菌，直径为0.7-1.0 mu m，并且始终发生在两个至四个细胞的簇中。脉冲ZC-1（T）的透析敏感性实验和分析表明存在蛋白质细胞壁。 ZC-1（T）菌株使用甲醇，甲胺和三甲胺作为甲烷的底物。最佳生长时间约为5小时，在6.0-6.5、65摄氏度，0.3-0.5 m NaCl和0.05-0.20 m mgCl2时发生。该生物的DNA G+C含量为56 mol％。对16S rRNA基因序列的分析和ZC-1（t）菌株MCRA基因的推断氨基酸序列表明，它与甲烷酸科的家族成员特别相关（分别为90.6和76.6％的序列相似性）。但是，ZC-1（T）菌株未能用乙酸盐作为甲烷生成的底物生长，这是甲藻科家族的特征。基于这些表型和系统发明特征，提出了ZC-1（T）菌株代表一种新颖的属和物种，其名称为甲壳虫shengliensis gen。 11月，sp。十一月。提出了。类型应变为ZC-1（T）（= CGMCC 1.5056（t）= DSM 18856（t））。甲皮菌科。十一月。也提出了。
A thermophilic, methylotrophic methanogen, strain ZC-1(T), was isolated from the Shengli oilfield, China. Cells of strain ZC-1(T) were motile cocci, 0.7-1.0 mu m in diameter and always occurred in clusters of two to four cells. Lysis-susceptibility experiments and analysis of transmission electron micrographs of strain ZC-1(T) suggested the presence of a proteinaceous cell wall. Strain ZC-1(T) used methanol, methylamine and trimethylamine as substrates for methanogenesis. Optimal growth, with a doubling time of around 5 h, occurred at pH 6.0-6.5, 65 degrees C, 0.3-0.5 M NaCl and 0.05-0.20 M MgCl2. The DNA G+C content of this organism was 56 mol%. Analysis of 16S rRNA gene sequence and the inferred amino acid sequence of the mcrA gene of strain ZC-1(T) indicated that it is related specifically to members of the family Methanosaetaceae (90.6 and 76.6% sequence similarity, respectively). However, strain ZC-1(T) failed to grow with acetate as substrate for methanogenesis, which is a special characteristic of the family Methanosaetaceae. Based on these phenotypic and phylogenic characteristics, strain ZC-1(T) is proposed to represent a novel genus and species, for which the name Methermicoccus shengliensis gen. nov., sp. nov. is proposed. The type strain is ZC-1(T) (=CGMCC 1.5056(T) = DSM 18856(T)). Methermicoccaceae fam. nov. is also proposed.