Solution structure of an extracellular domain containing the WSxWS motif of the granulocyte colony-stimulating factor receptor and its interaction with ligand
Solution structure of an extracellular domain containing the WSxWS motif of the granulocyte colony-stimulating factor receptor and its interaction with ligand
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含有粒细胞集落刺激因子受体 WSxWS 基序的胞外结构域的溶液结构及其与配体的相互作用
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
Y. Ota
中科院分区：
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作者：
K. Yamasaki;Shoko Naito;H. Anaguchi;T. Ohkubo;Y. Ota
我们已经确定了粒细胞集落刺激因子 (G-CSF) 受体配体结合域的 NMR 结构，其中包含高度保守的 WSxWS 基序。该结构域由七个 β 链组成，具有在几种细胞因子受体中看到的纤连蛋白 III 型拓扑结构。具有和不具有 G-CSF 的 15N 标记结构域的光谱之间的比较表明，主要配体识别位点位于 WSxWS 序列上游的 FG 环上，而不是主要用于生长激素系统的 BC 环上。 WSxWS 残基被认为有助于配体识别和 G-CSF 受体的蛋白质结构。
We have determined the NMR structure of a ligand-binding domain of the granulocyte colony-stimulating factor (G-CSF) receptor, containing the highly conserved WSxWS motif. The domain consists of seven β-strands with the f ibronectin type III-like topology seen in several cytokine receptors. Comparisons between the spectra of the 15N-labelled domain with and without G-CSF indicate that the major ligand-recognition site is on the FG loop just upstream of the WSxWS sequence, and not on the BC loop which is mainly used in the growth hormone system. The WSxWS residues are suggested to contribute to ligand-recognition and to the protein architecture of the G-CSF receptor.