Natural skip oviposition of the mosquito Aedes aegypti indicated by codominant genetic markers
Natural skip oviposition of the mosquito Aedes aegypti indicated by codominant genetic markers
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DOI：
10.1046/j.1365-2915.2003.00424.x
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发表时间：
2003-06-01
影响因子：
1.9
通讯作者：
Severson, DW
中科院分区：
文献类型：
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作者：
Colton, YM;Chadee, DD;Severson, DW
本研究探讨了使用共显性限制性片段长度多态性（RFLP）标记估计的兄弟姐妹家庭的数量内和之间的蚊子埃及伊蚊（L）（双翅目：蚊科）的产卵地点。使用基于个体之间共享的等位基因的成对相关性（r（xy））计算进行估计。8个实验室蚊子家庭的基因型确定在6个RFLP位点和观察到的等位基因频率被用来产生模拟分布的r（XY）从全同胞和无关的个人对。两两r（xy）分布的平均值之间的中点（mp）被用来区分全同胞家庭无关的家庭。集群的r（xy）值高于mp值的个人被分组为假定的兄弟姐妹家庭。通过计算已知实验室全同胞和父方半同胞家庭的所有成对比较的实际r（xy），然后通过upgma聚类分析对同胞家庭进行分组，来测试该方法。然后，该技术被用于估计在西印度群岛特立尼达岛的三个地点收集的野生捕获的蚊子的同胞。从田间种群中，估计35个家庭中的122个个体测试，平均6.2家庭每个容器。19个预测的家庭成员聚集在多个容器的群体，提供跳产卵行为在Ae的分子证据。埃及雌性，其中个体雌性在一个以上的容器中产卵。
This study examines the use of codominant restriction fragment length polymorphism (RFLP) markers to estimate the number of sibling families found within and among oviposition sites used by the mosquito Aedes aegypti (L) (Diptera: Culicidae). Estimates were made using pairwise relatedness (r (xy)) calculations based on alleles shared between individuals. Genotypes for eight laboratory mosquito families were determined at six RFLP loci and the observed allele frequencies were used to generate simulated distributions of r (xy) from full-sibling and unrelated pairs of individuals. The midpoint (mp) between the means of the pairwise r(xy) distributions was used to discriminate full-sibling families from unrelated families. Clusters of individuals with r(xy) values higher than the mp value were grouped as putative sibling families. This method was tested by calculating actual r(xy) for all pairwise comparisons of the known laboratory full-sibling and paternal half-sibling families, followed by upgma cluster analysis to group sibling families. The technique was then used for sibling estimations on wild caught mosquitoes collected at three locations in Trinidad, West Indies. From field populations, 35 families were estimated among 122 individuals tested with an average of 6.2 families per container. Members of 19 predicted families clustered as groups across multiple containers, providing molecular evidence for skip-oviposition behaviour in Ae. aegypti females, whereby individual females oviposit in more than one container.