DETECTION OF CARBONYL FUNCTIONS IN PHOSPHOLIPIDS OF LIVER-MICROSOMES IN CCL4-POISONED AND BRCCL3-POISONED RATS
DETECTION OF CARBONYL FUNCTIONS IN PHOSPHOLIPIDS OF LIVER-MICROSOMES IN CCL4-POISONED AND BRCCL3-POISONED RATS
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DOI：
10.1016/0005-2760(82)90292-2
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
COMPORTI, M
中科院分区：
文献类型：
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作者：
BENEDETTI, A;FULCERI, R;COMPORTI, M
由于不饱和脂肪酸的过氧化裂解可导致羰基化合物的释放或酰基残基中羰基官能团的形成，因此在体内条件（CCl 4和BrCCl 3中毒）中寻找肝微粒体磷脂中存在羰基的证据，其中先前已证明肝内质网脂质过氧化。中毒动物肝微粒体经2，4-二硝基苯肼处理的磷脂的分光光度检查显示吸收光谱与各种羰基的二硝基苯腙所观察到的吸收光谱相似。类似的光谱，虽然从定量的角度放大，也观察到与2，4-二硝基苯肼处理过氧化的肝微粒体的磷脂在NADPH-Fe依赖性系统。微粒体脂质过氧化的时程研究表明，肝微粒体磷脂中的2，4-二硝基苯肼反应基团（羰基官能团）的量随孵育时间增加，并与孵育混合物中形成的丙二醛的量相关。4-羟基壬烯醛的生成动力学与丙二醛的生成动力学相似。在两种体内条件（CCl 4和BrCCl 3中毒）中，微粒体磷脂中羰基官能团的量（在BrCCl 3中毒的动物中更高）接近于在体外条件中发现的量，在体外条件中，脂质过氧化由6 μ M Fe 2+诱导。膜磷脂中羰基功能的形成可能的病理意义进行了讨论。
Since the peroxidative cleavage of unsaturated fatty acids can result in the release of carbonyl compounds or the formation of carbonyl functions in the acyl residues, evidence for the presence of carbonyl groups in liver microsomal phospholipids was searched for in in vivo conditions (CCl4 and BrCCl3 intoxications) in which peroxidation of lipids of hepatic endoplasmic reticulum had been previously demonstrated. The spectrophotometric examination of 2,4-dinitrophenylhydrazine-treated phospholipids of liver microsomes from the intoxicated animals showed absorption spectra similar to those observed for the dinitrophenylhydrazones of various carbonyls. Similar spectra, although magnified from a quantitative point of view, were also observed with 2,4-dinitrophenylhydrazine-treated phospholipids of liver microsomes peroxidized in the NADPH-Fe-dependent system. A time-course study of microsomal lipid peroxidation showed that the amount of 2,4-dinitrophenylhydrazine-reacting groups (carbonyl functions) in phospholipids of liver microsomes increased with incubation time and is correlated to the amount of malonic dialdehyde formed in the incubation mixture. The kinetics of the production of 4-hydroxynonenal was similar to that of malonic dialdehyde formation. In both in vivo conditions (CCl4 and BrCCl3 intoxications) the amount of carbonyl functions in microsomal phospholipids, which was higher in the BrCCl3-intoxicated animals, was close to that found in the vitro condition in which lipid peroxidation is induced by 6 .mu.M Fe2+. The possible pathological significance of formation of carbonyl functions in membrane phospholipids is discussed.