Relationship between O-antigen subtypes, bacterial surface structures and O-antigen gene clusters in Escherichia coli O123 strains carrying genes for Shiga toxins and intimin
Relationship between O-antigen subtypes, bacterial surface structures and O-antigen gene clusters in Escherichia coli O123 strains carrying genes for Shiga toxins and intimin
复制标题
携带志贺毒素和 intimin 基因的大肠杆菌 O123 菌株中 O 抗原亚型、细菌表面结构和 O 抗原基因簇之间的关系
DOI：
10.1099/jmm.0.46775-0
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发表时间：
2007-02-01
影响因子：
3
通讯作者：
Feng, Lu
中科院分区：
文献类型：
--
作者：
Beutin, Lothar;Wang, Quan;Feng, Lu
Escherichia coli O123 strains express a broad spectrum of phenotypes, H serotypes and virulence markers and are able to colonize and to cause disease in different hosts including humans. In this study, two subtypes of E coli O123 antigen (group I and group II) have been identified based on their cross-reactions with other E coli O antigens. Investigation of the relationship between O123 group I and group II strains by O serotyping and Fourier transform infrared spectroscopy of whole bacteria revealed surface structural differences between these two groups of E coli O123 strains. Nucleotide sequence analysis of the O-antigen gene clusters of two E coli O123 strains representing O123 group I and group II revealed no change at the amino acid level. These findings indicate that the differences in the surface structures of group I and group II strains are not related to genetic heterogeneity in their C-antigen gene clusters. A PCR assay based on O123 antigen-specific wzx and wzy genes was developed and found to be suitable for reliable detection of all subtypes of E coli O123 strains, which bears an advantage over traditional serological detection.
