Comparison of ELISA and SPR biosensor technology for the detection of paralytic shellfish poisoning toxins
Comparison of ELISA and SPR biosensor technology for the detection of paralytic shellfish poisoning toxins
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DOI：
10.1016/j.jchromb.2009.10.023
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发表时间：
2009-12-15
影响因子：
3
通讯作者：
Elliott, Christopher T.
中科院分区：
文献类型：
--
作者：
Campbell, Katrina;Huet, Anne-Catherine;Elliott, Christopher T.
开发了用于检测麻痹性贝类中毒（PSP）毒素的酶标记免疫吸附测定（ELISA）和表面等离子共振（SPR）生物传感器测定，并进行了比较评估。用于两种检测形式的多克隆抗体 (BC67) 在新西兰白兔中针对石房蛤毒素-杰法明-BSA 产生。每种测定形式都被设计为抑制测定。使用两种简单的快速提取程序对每种方法对贝类样品 (n = 54) 进行评估，并与 AOAC 高效液相色谱 (HPLC) 和小鼠生物测定 (MBA) 进行比较。每种检测形式的结果与 HPLC 和 MBA 方法相当，并证明对 PSP 毒素具有高灵敏度和广泛特异性的抗体可以应用于不同的免疫学技术。选择的方法将取决于最终用户的需求。与 ELISA 相比，SPR 生物传感器减少了体力劳动，操作简单，易于提取样品，并且具有卓越的实时半定量分析能力，这些都是使该技术适用于高通量监测装置的关键特征。 (C) 2009 Elsevier B.V. 保留所有权利。
An enzyme labeled immunosorbent assay (ELISA) and surface plasmon resonance (SPR) biosensor assay for the detection of paralytic shellfish poisoning (PSP) toxins were developed and a comparative evaluation was performed. A polyclonal antibody (BC67) used in both assay formats was raised to saxitoxin-jeffamine-BSA in New Zealand white rabbits. Each assay format was designed as an inhibition assay. Shellfish samples (n = 54) were evaluated by each method using two simple rapid extraction procedures and compared to the AOAC high performance liquid chromatography (HPLC) and the mouse bioassay (MBA). The results of each assay format were comparable with the HPLC and MBA methods and demonstrate that an antibody with high sensitivity and broad specificity to PSP toxins can be applied to different immunological techniques. The method of choice will depend on the end-users needs. The reduced manual labor and simplicity of operation of the SPR biosensor compared to ELISA, ease of sample extraction and superior real time semi-quantitative analysis are key features that could make this technology applicable in a high-throughput monitoring unit. (C) 2009 Elsevier B.V. All rights reserved.