T2 and Apparent Diffusion Coefficient of MRI Reflect Maturation of Tissue-Engineered Auricular Cartilage Subcutaneously Transplanted in Rats
T2 and Apparent Diffusion Coefficient of MRI Reflect Maturation of Tissue-Engineered Auricular Cartilage Subcutaneously Transplanted in Rats
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DOI：
10.1089/ten.tec.2015.0291
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发表时间：
2016-05-01
影响因子：
3
通讯作者：
Hoshi, Kazuto
中科院分区：
文献类型：
--
作者：
Fujihara, Yuko;Nitta, Naotaka;Hoshi, Kazuto
在软骨再生医学中，自体软骨细胞移植（ACI）已应用于临床关节软骨部分缺损或隆鼻。为了使ACI治疗更有效和普遍，非侵入性评估移植结构质量的方法是必要的。在这项研究中，我们比较了几种非侵入性方法评估含有可生物降解聚合物支架的组织工程耳软骨成熟的功效。我们首先将由人耳廓软骨细胞、去端胶原凝胶和聚乳酸（PLLA）多孔支架组成的组织工程软骨皮下移植到无胸腺裸鼠背部。移植后8周，大鼠进行了检查，磁共振成像（MRI），X射线和超声作为非侵入性的方式。然后，切除的结构进行了检查，通过组织学和生化分析，包括甲苯胺蓝（TB）染色，糖胺聚糖含量，和酶联免疫吸附试验的II型胶原。其中MRI的横向弛豫时间（T2）和表观扩散系数与组织学和生化结果显示出相当高的相关性，这表明这些可以有效地检测组织工程化耳廓软骨的成熟。由于这些非侵入性的方式将实现组织工程软骨成熟的时间过程分析，本研究提供了一个实质性的见解，以提高组织工程软骨的质量，导致软骨再生医学的质量和技术的改进。
In cartilage regenerative medicine, autologous chondrocyte implantation (ACI) has been applied clinically for partial defects of joint cartilage or nasal augmentation. To make treatment with ACI more effective and prevalent, modalities to evaluate the quality of transplanted constructs noninvasively are necessary. In this study, we compared the efficacy of several noninvasive modalities for evaluating the maturation of tissue-engineered auricular cartilage containing a biodegradable polymer scaffold. We first transplanted tissue-engineered cartilage consisting of human auricular chondrocytes, atelocollagen gel, and a poly-l-lactic acid (PLLA) porous scaffold subcutaneously into the back of athymic nude rats. Eight weeks after transplantation, the rats were examined by magnetic resonance imaging (MRI), X-ray, and ultrasound as noninvasive modalities. Then, the excised constructs were examined by histological and biochemical analysis including toluidine blue (TB) staining, glycosaminoglycans content, and enzyme-linked immunosorbent assay of type II collagen. Among the modalities examined, transverse relaxation time (T2) and apparent diffusion coefficient of MRI showed quite a high correlation with histological and biochemical results, suggesting that these can effectively detect the maturation of tissue-engineered auricular cartilage. Since these noninvasive modalities would realize time-course analysis of the maturation of tissue-engineered auricular cartilage, this study provides a substantial insight for improving the quality of tissue-engineered cartilage, leading to improvement of the quality and technique in cartilage regenerative medicine.