Different time courses of recovery after poisoning with botulinum neurotoxin serotypes A and E in humans
Different time courses of recovery after poisoning with botulinum neurotoxin serotypes A and E in humans
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DOI：
10.1016/s0304-3940(98)00775-7
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发表时间：
1998-11-13
影响因子：
2.5
通讯作者：
Montecucco, C
中科院分区：
文献类型：
--
作者：
Eleopra, R;Tugnoli, V;Montecucco, C
肉毒杆菌毒素血清型 A 和 E（BoNT/A 和 /E）可裂解突触体相关蛋白 25 (SNAP-25) 的羧基末端，分别去除 9 个和 26 个残基。为了研究同一靶分子对这些损伤的影响，11 名志愿者在一只脚的趾短伸肌 (EDB) 肌肉中注射了 3 IU BoNT/A，在对侧一只脚中注射了 3 IU BoNT/E。此外，七名志愿者也同样注射了 BoNT/A + BoNT/E 的混合物。在不同的时间间隔测量复合肌肉动作电位（CMAP）并计算CMAP的百分比变化（%CMAP）。出乎意料的是，注射 BoNT/E 后观察到 %CMAP 恢复得更快。双重中毒的 EBD 肌肉恢复情况与 BoNT/E 类似。因此，BoNT/E 引起的 SNAP-25 分子的较大缺失会导致更快的功能恢复。 (C) 1998 Elsevier Science Ireland Ltd. 保留所有权利。
Botulinum toxin serotypes A and E (BoNT/A and /E) cleave the carboxy-terminus of synaptosomal associated protein-25 (SNAP-25) removing nine and 26 residues, respectively. To investigate the effect of these lesions of the same target molecule, 11 volunteers were injected with 3 IU of BoNT/A in the extensor digitorum brevis (EDB) muscle of one foot and with 3 IU of BoNT/E in the contralateral one. In addition, seven volunteers were similarly injected with mixtures of BoNT/A + BoNT/E. Compound muscular action potential (CMAP) was measured at different time intervals and the percentage variation of CMAP (%CMAP) was calculated. Unexpectedly, a much faster recovery of %CMAP after BoNT/E injections was observed. Double poisoned EBD muscles recovered similarly to BoNT/E. So, a larger deletion of the SNAP-25 molecule caused by BoNT/E leads to a faster functional recovery. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.