Immunologic responses of Pacific hagfish. 3. Serum antibodies to cellular antigens.
Immunologic responses of Pacific hagfish. 3. Serum antibodies to cellular antigens.
复制标题
太平洋盲鳗的免疫反应。
作者：
D. Linthicum;W. Hildemann
盲鳗血清含有天然存在的凝集素，其与各种脊椎动物红细胞上的异源非福斯曼抗原发生不同程度的反应。这些通常活性较低的抗体被发现存在于巨球蛋白 (IgM) 部分中，但在 56°C 加热血清 30 分钟后变得无法检测到。如果采取适当的免疫策略，通常可以诱导针对绵羊红细胞的特异性抗体产生。在 18° 的 70 天期间，每 14 天接受体重调整剂量的盲鳗显示出比血凝滴度增加 2 3 至 2 5 倍。诱导的抗体具有热稳定性，并且对绵羊细胞抗原表现出高度的特异性。色谱和免疫电泳分析表明，天然存在的和诱导的抗体活性仅限于与高等脊椎动物的 IgM 相似但可区分的主要巨球蛋白部分。尽管盲鳗处于“最原始的脊椎动物”状态，但至少能够旺盛地产生针对某些细胞和可溶性抗原的抗体。其免疫遗传库和免疫记忆的范围有待进一步研究。
Hagfish serum contains naturally-occurring agglutinins which react to different degrees with heterogenetic, but non-Forssman antigens on the erythrocytes of various vertebrates. These antibodies of generally low activity were found to reside in the macroglobulin (IgM) fraction, but became undetectable after heating serum at 56°C for 30 min. Specific production of antibodies against sheep erythrocytes was regularly inducible, given suitable tactics of immunization. Hagfish which received weight-adjusted dosages every 14 days over a 70-day period at 18° showed 2 3 - to 2 5 -fold rises in specific hemagglutination titers. Induced antibodies were heat stable and displayed a high degree of specificity for sheep cell antigens. Chromatographic and immunoelectrophoretic analyses showed the naturally-occurring and induced antibody activity to be limited to a major macroglobulin fraction similar to but distinguishable from the IgM of higher vertebrates. Hagfish, despite their ‘most primitive vertebrate” status, are capable of vigorous production of antibodies to at least certain cellular and soluble antigens. The extent of their immunogenetic library and immunologic memory invites further investigation.