PSORIASIN BINDS CALCIUM AND IS UP-REGULATED BY CALCIUM TO LEVELS THAT RESEMBLE THOSE OBSERVED IN NORMAL SKIN
PSORIASIN BINDS CALCIUM AND IS UP-REGULATED BY CALCIUM TO LEVELS THAT RESEMBLE THOSE OBSERVED IN NORMAL SKIN
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DOI：
10.1111/1523-1747.ep12395202
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发表时间：
1994-09-01
影响因子：
6.5
通讯作者：
CELIS, JE
中科院分区：
文献类型：
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作者：
HOFFMANN, HJ;OLSEN, E;CELIS, JE
最近，我们描述了一个小分子量的蛋白质称为银屑病，显示序列相似性与S100钙结合蛋白，高度上调银屑病表皮以及在初级人类角质形成细胞进行异常分化。在这里，我们提出的证据表明，天然和重组银屑病结合钙，钙覆盖测定判断，它包含所有的序列特征的S100家族。此外，[S-35]-甲硫氨酸标记实验表明，银屑病蛋白合成上调2 mM Ca++（比Ca++/对照组在88小时= 2.56）的水平，类似于从正常皮肤中获得的未分级角质形成细胞群体中观察到的水平。
Recently, we described a small molecular weight protein termed psoriasin that showed sequence similarity with the S100 calcium-binding proteins and that highly upregulated in psoriatic epidermis as well as in primary human keratinocytes undergoing abnormal differentiation. Here we present evidence showing that natural and recombinant psoriasin binds calcium, as judged by the calcium overlay assay, and that it contains all the sequence features characteristic of the S100 family. Furthermore, [S-35]-methionine labeling experiments showed that psoriasin synthesis is upregulated by 2 mM Ca++ (ratio Ca++/control at 88 h = 2.56) to levels that resemble those observed in unfractionated keratinocyte populations obtained from normal skin.