Detection and Characterization of Estrogen Receptor Beta Expression in the Brain with Newly Developed Transgenic Mice
Detection and Characterization of Estrogen Receptor Beta Expression in the Brain with Newly Developed Transgenic Mice
复制标题
DOI：
10.1016/j.neuroscience.2020.04.047
复制
发表时间：
2020-05
期刊：
影响因子：
3.3
通讯作者：
Shoko Sagoshi;Sho Maejima;Masahiro Morishita;Satoshi Takenawa;Akito Otubo;K. Takanami;T. Sakamoto;H. Sakamoto;S. Tsukahara;S. Ogawa
中科院分区：
文献类型：
--
作者：
Shoko Sagoshi;Sho Maejima;Masahiro Morishita;Satoshi Takenawa;Akito Otubo;K. Takanami;T. Sakamoto;H. Sakamoto;S. Tsukahara;S. Ogawa
两种类型的核雌激素受体，ERα和ERβ，已被证明是不同的参与各种类型的行为的调节。由于缺乏鉴定ERβ表达的工具，ERβ表达细胞的详细解剖分布和神经化学特征以及与ERα的细胞共表达仍不清楚。将RFP插入ERβ BAC启动子下游，构建了ERβ-RFPtg转基因小鼠。我们通过以下方式验证RFP信号为ERβ：（1）通过荧光激活细胞分选收集的RFP表达细胞中的高ERβ mRNA水平;（2）室旁核（PVN）中ERβ mRNA和RFP蛋白的共定位。ERβ-RFP在PVN、内侧视前区（MPOA）、终纹床核、内侧杏仁核（MeA）和中缝背核（DRN）等核团有较强信号。在MPOA和MeA中，鉴定了三种类型的细胞群：同时表达ERα和ERβ的细胞群，以及仅表达ERα或ERβ的细胞群。大多数PVN和DRN细胞仅表达ERβ-RFP。此外，ERβ-RFP阳性细胞在PVN中共表达催产素，在DRN中共表达色氨酸羟化酶2和孕酮受体。在MeA中，部分ERβ-RFP阳性细胞共表达催产素受体。这些发现共同表明，ERβ-RFTg小鼠可以成为未来研究ERβ在雌激素调节社会行为中功能的有力工具。
Two types of nuclear estrogen receptors, ERα and ERβ, have been shown to be differentially involved in the regulation of various types of behaviors. Due to a lack of tools for identifying ERβ expression, detailed anatomical distribution and neurochemical characteristics of ERβ expressing cells and cellular co-expression with ERα remain unclear. We have generated transgenic mice ERβ-RFPtg, in which RFP was inserted downstream of ERβ BAC promotor. We verified RFP signals as ERβ by confirming: (1) high ERβ mRNA levels in RFP-expressing cells collected by fluorescence-activated cell sorting; and (2) co-localization of ERβ mRNA and RFP proteins in the paraventricular nucleus (PVN). Strong ERβ-RFP signals were found in the PVN, medial preoptic area (MPOA), bed nucleus of the stria terminalis, medial amygdala (MeA), and dorsal raphe nucleus (DRN). In the MPOA and MeA, three types of cell populations were identified; those expressing both ERα and ERβ, and those expressing exclusively either ERα or ERβ. The majority of PVN and DRN cells expressed only ERβ-RFP. Further, ERβ-RFP positive cells co-expressed oxytocin in the PVN, and tryptophan hydroxylase 2 and progesterone receptors in the DRN. In the MeA, some ERβ-RFP positive cells co-expressed oxytocin receptors. These findings collectively suggest that ERβ-RFPtgmice can be a powerful tool for future studies on ERβ function in the estrogenic regulation of social behaviors.