INHIBITION OF PROTEIN SYNTHESIS IN RETICULOCYTES BY ANTIBIOTICS .I. EFFECTS ON POLYSOMES
INHIBITION OF PROTEIN SYNTHESIS IN RETICULOCYTES BY ANTIBIOTICS .I. EFFECTS ON POLYSOMES
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DOI：
10.1016/0005-2787(66)90043-8
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发表时间：
1966-01-01
期刊：
影响因子：
--
通讯作者：
BAGLIONI, C
中科院分区：
文献类型：
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作者：
COLOMBO, B;FELICETTI, L;BAGLIONI, C
已经测试了几种抗生素对蛋白质合成的抑制作用：放线菌酮、链维他霉素A和帕他霉素在完整网织红细胞和从网织红细胞制备的无细胞系统中的合成。司帕霉素仅在无细胞系统中抑制蛋白质合成。结构上相关的环己酰亚胺和链维他霉素A可能具有共同的作用机制。放线菌酮的抑制作用是可逆的，而链维他霉素A的抑制作用似乎是完全不可逆的。放线菌酮和链维A霉素的行为差异似乎是由于链维A霉素不扩散出网织红细胞，即使它很容易进入这些细胞。环己酰亚胺和链维A菌素不改变网织红细胞裂解物的多核糖体模式，也不引起不完整肽链的释放。帕他霉素降解多核糖体，缓慢释放推测已完成的肽链。
Several antibiotics have been tested for inhibition of protein synthesis: cycloheximide, streptovitacin A and pactamycin synthesis in intact reticulocytes and in a cell-free system prepared from reticulocytes. Sparsomycin inhibits protein synthesis in the cell-free system only. Cycloheximide and streptovitacin A, which are structurally related, presumably have a common mechanism of action. The inhibition by cycloheximide is reversible,while that by streptovitacin A seems to be completely irreversible. The difference in behavior of cycloheximide and streptovitacin A seems to be attributable to the fact that streptovitacin A does not diffuse out of reticulocytes, even though it readily enters these cells. Cycloheximide and streptovitacin A do not change the polysome pattern of reticulocyte lysate, nor cause the release of incomplete peptide chains. Pactamycin degrades the polysomes with the slow release of presumably completed peptide chains.