Identification of Lipoteichoic Acid as a Ligand for Draper in the Phagocytosis of Staphylococcus aureus by Drosophila Hemocytes
Identification of Lipoteichoic Acid as a Ligand for Draper in the Phagocytosis of Staphylococcus aureus by Drosophila Hemocytes
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DOI：
10.4049/jimmunol.0901032
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发表时间：
2009-12-01
影响因子：
4.4
通讯作者：
Shiratsuchi, Akiko
中科院分区：
文献类型：
--
作者：
Hashimoto, Yumi;Tabuchi, Yukichika;Shiratsuchi, Akiko
吞噬作用是抵抗细菌感染的细胞免疫的核心。在哺乳动物中，调理素依赖和非依赖的吞噬机制似乎存在于果蝇。虽然已经报道了果蝇吞噬作用的候选受体，但它们如何直接或间接识别细菌仍有待阐明。我们在筛选细胞壁结构有缺陷的突变株时，寻找果蝇血细胞吞噬所需的金黄色葡萄球菌基因。鉴定的基因包括ItaS，它编码一种负责合成脂磷壁酸的酶。Itas依赖的S.金黄色葡萄球菌需要受体德雷珀，但不需要Eater或尼姆罗德C1，而缺乏德雷珀的果蝇对S.金黄色葡萄球菌没有改变不道德的免疫反应。最后，脂磷壁酸结合到德雷珀的细胞外区域。我们认为脂磷壁酸在S.金黄色葡萄球菌的果蝇血细胞和吞噬消除入侵的细菌是苍蝇生存的感染。免疫学杂志，2009，183：7451-7460。
Phagocytosis is central to cellular immunity against bacterial infections. As in mammals, both opsonin-dependent and -independent mechanisms of phagocytosis seemingly exist in Drosophila. Although candidate Drosophila receptors for phagocytosis have been reported, how they recognize bacteria, either directly or indirectly, remains to be elucidated. We searched for the Staphylococcus aureus genes required for phagocytosis by Drosophila hemocytes in a screening of mutant strains with defects in the structure of the cell wall. The genes identified included ItaS, which encodes an enzyme responsible for the synthesis of lipoteichoic acid. ItaS-dependent phagocytosis of S. aureus required the receptor Draper but not Eater or Nimrod C1, and Draper-lacking flies showed reduced resistance to a septic infection of S. aureus without a change in a Immoral immune response. Finally, lipoteichoic acid bound to the extracellular region of Draper. We propose that lipoteichoic acid serves as a ligand for Draper in the phagocytosis of S. aureus by Drosophila hemocytes and that the phagocytic elimination of invading bacteria is required for flies to survive the infection. The Journal of Immunology, 2009, 183: 7451-7460.