The testate lobose amoebae (order Arcellinida Kent, 1880) finally find their home within Amoebozoa
The testate lobose amoebae (order Arcellinida Kent, 1880) finally find their home within Amoebozoa
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DOI：
10.1016/j.protis.2005.03.002
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发表时间：
2005-08-01
期刊：
影响因子：
2.5
通讯作者：
Pawlowski, J
中科院分区：
文献类型：
--
作者：
Nikolaev, SI;Mitchell, EAD;Pawlowski, J
球状变形虫(Arcellinida Kent，1880)在所有水生和陆地栖息地中都很常见，但它们是最后几个没有在生命树上找到位置的单细胞真核生物之一。形态方法不允许确定该群体的进化起源或证明其单调性。为了解决这些具有挑战性的问题，我们分析了7个雄性大叶阿米巴的部分小亚基核糖体RNA(SSU RRNA)基因，这些阿米巴来自3个亚目中的2个和属于Arcellinida的13个科中的7个。我们的数据支持该目的单系性，并清楚地确立了它在变形虫中的地位，它是由变形科和哈特曼尼利科组成的支系的姐妹群。来自两个物种的完整SSU rRNA基因序列和来自一个物种的肌动蛋白部分序列证实了这一位置。我们的系统发育分析包括了大叶阿米巴的所有序列谱系的代表，表明在变形虫的进化过程中只出现过一次严格的测试，并允许重新解释用于Arcellinida系统学的一些形态特征。(C)2005年，爱思唯尔股份有限公司出版。
Testate lobose amoebae (order Arcellinida Kent, 1880) are common in all aquatic and terrestrial habitats, yet they are one of the last higher taxa of unicellular eukaryotes that has not found its place in the tree of life. The morphological approach did not allow to ascertain the evolutionary origin of the group or to prove its monophyly. To solve these challenging problems, we analyzed partial small-subunit ribosomal RNA (SSU rRNA) genes of seven testate lobose amoebae from two out of the three suborders and seven out of the 13 families belonging to the Arcellinida. Our data support the monophyly of the order and clearly establish its position among Amoebozoa, as a sister-group to the clade comprising families Amoebidae and Hartmannellidae. Complete SSU rRNA gene sequences from two species and a partial actin sequence from one species confirm this position. Our phylogenetic analyses including representatives of all sequenced lineages of lobose amoebae suggest that a rigid test appeared only once during the evolution of the Amoebozoa, and allow reinterpretation of some morphological characters used in the systematics of Arcellinida. (c) 2005 Published by Elsevier GmbH.