Clinical isolates of GB virus type C vary in their ability to persist and replicate in peripheral blood mononuclear cell cultures
Clinical isolates of GB virus type C vary in their ability to persist and replicate in peripheral blood mononuclear cell cultures
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DOI：
10.1016/s0042-6822(03)00585-3
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发表时间：
2003-11-25
期刊：
影响因子：
3.7
通讯作者：
Stapleton, JT
中科院分区：
文献类型：
--
作者：
George, SL;Xiang, JH;Stapleton, JT
GB病毒C/G型肝炎病毒(GBV-C)在体外复制效率低且不一致。本研究采用基于外周血单个核细胞(PBMC)的培养方法对GBV-C临床分离株进行了鉴定。分离株持续复制的能力各不相同，在没有PHA/IL-2刺激的情况下生长的细胞产量增加。测定了一个复制良好的分离物的推导多蛋白序列(GenBank AY196904)，并与20个全长GBV-C序列进行了比较。在确认的16个独特的氨基酸多态中，有14个位于与干扰素抵抗和RNA复制相关的非结构蛋白的编码区。这些数据表明，临床分离的GBV-C在培养中的持久力各不相同，不需要PHA/IL-2刺激，关键调节区的序列变异性可能会影响PBMC培养的生长。由于GBV-C似乎在混合感染模型中抑制艾滋病毒复制，这些研究应该有助于确定这种相互作用的机制。(C)2003 Elsevier Inc.保留所有权利。
GB virus C/hepatitis G virus (GBV-C) replication in vitro is inefficient and inconsistent. In this study, clinical isolates of GBV-C were evaluated using peripheral blood mononuclear cell (PBMC) based culture methods. Isolates varied consistently in their ability to persistently replicate, and yield increased in cells grown without PHA/IL-2 stimulation. The deduced polyprotein sequence of an isolate that replicated well was determined (GenBank AY196904) and compared to 20 full-length GBV-C sequences. Fourteen of the 16 unique amino acid polymorphisms identified were in the coding regions for nonstructural proteins associated with interferon resistance and RNA replication. These data indicate that clinical GBV-C isolates vary in their ability to persist in culture, do not require PHA/IL-2 stimulation, and that sequence variability in key regulatory regions may affect growth in PBMC cultures. Since GBV-C appears to inhibit HIV replication in a coinfection model, these studies should facilitate determination of the mechanism of this interaction. (C) 2003 Elsevier Inc. All rights reserved.