Colonization of gnotobiotic piglets by Helicobacter pylori deficient in two flagellin genes
Colonization of gnotobiotic piglets by Helicobacter pylori deficient in two flagellin genes
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DOI：
10.1128/iai.64.7.2445-2448.1996
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发表时间：
1996-07-01
影响因子：
3.1
通讯作者：
Krakowka, S
中科院分区：
文献类型：
--
作者：
Eaton, KA;Suerbaum, S;Krakowka, S
幽门螺杆菌具有两种鞭毛蛋白分子，主要种类FlaA和少量表达的FlaB。本研究试图确定一种或两种鞭毛蛋白是否是H.幽门。对6窝36头无菌仔猪分别接种4株H. pylori口服使用的细菌菌株是菌株N6，野生型，其产生FlaA和FlaB并且是完全运动的; N6 flaB：：km，其产生FlaA但不产生FlaB并且是弱运动的; N6 flaA：：km，其表达FlaB但不表达FLaA并且是非运动的;以及N6 flaA：：cat/flaB：：km，其既不产生鞭毛蛋白也是非运动的。菌株N6在所有仔猪中定殖，并在接种后持续2、4和10天。N6 flaA：：km和N6 flaB：：km定殖时间分别为2天和4天，但不超过10天，定殖能力较弱。N6 flaA：：cat/flaB：：km定殖2天，但在接种后不持续4或10天。这些发现表明，这两种鞭毛蛋白是H.幽门。在不存在任何一种鞭毛蛋白的情况下，定植长达4天是可能的，但两者都不存在。
Helicobacter pylori possesses two flagellin molecules, FlaA, the major species, and FlaB, which is expressed in minor amounts. This study sought to determine if one or both flagellin species are necessary for colonization or persistence by H. pylori. Thirty-six gnotobiotic piglets from six litters were given one of four isogenic strains of H. pylori orally. The bacterial strains used were strain N6, the wild type, which produced both FlaA and FlaB and was fully motile; N6flaB::km, which produced FlaA but not FlaB and was weakly motile; N6flaA::km, which expressed FlaB but not FLaA and was nonmotile; and N6flaA::cat/flaB::km, which produced neither flagellin and was nonmotile. Strain N6 colonized all piglets and persisted for 2, 4, and 10 days after inoculation. Both N6flaA::km and N6flaB::km colonized for 2 and 4 but not 10 days, and colonization was weak. N6flaA::cat/flaB::km colonized for 2 days but did not persist for 4 or 10 days after inoculation. These findings demonstrate that both flagellin species are necessary for full colonization by H. pylori. Colonization for up to 4 days is possible in the absence of either flagellin species but not both.