Basophil depletion downregulates Schistosoma mansoni egg-induced granuloma formation
Basophil depletion downregulates Schistosoma mansoni egg-induced granuloma formation
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DOI：
10.1016/j.parint.2013.07.003
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发表时间：
2013-12-01
影响因子：
1.9
通讯作者：
Ohta, Nobuo
中科院分区：
文献类型：
--
作者：
Anyan, William K.;Seki, Takenori;Ohta, Nobuo
寄生虫感染期间寄生虫卵周围肉芽肿的形成被认为是由Th 2细胞因子控制的。然而，它仍然是有争议的细胞群体是负责的主机Th 2细胞依赖性肉芽肿的形成。嗜碱性粒细胞由于其产生大量IL-4的能力最近引起了人们的注意。因此，我们研究嗜碱性粒细胞是否在曼氏血吸虫虫卵诱导的肉芽肿形成中起重要作用。结合我们先前观察到的感染后7周脾脏嗜碱性粒细胞数量显著增加，使用抗mMCP 8单克隆抗体（mAb）的免疫组化染色显示嗜碱性粒细胞浸润在寄生虫卵周围形成的肉芽肿性病变中。为了更直接地检查嗜碱性粒细胞的作用，我们用抗CD 200 R3 mAb处理小鼠以消耗嗜碱性粒细胞。嗜碱性粒细胞的耗竭导致嗜碱性粒细胞数量减少，同时在感染后7周时虫卵肉芽肿形成下调。此外，我们观察到在肺肉芽肿模型中嗜碱性粒细胞耗竭小鼠中形成的虫卵肉芽肿的大小显著减小。总之，这些发现表明嗜碱性粒细胞是必不可少的S。曼氏虫卵诱导的肉芽肿形成，这可能是一个新的治疗目标，在改善血吸虫病的病理。(C)2013爱思唯尔爱尔兰有限公司版权所有。
Granuloma formation around parasite eggs during schistosomal infection is considered to be controlled by Th2 cytokines. However, it is still controversial which cell populations are responsible for the host Th2 cytokine-dependent granuloma formation. Basophils have recently attracted attention because of their ability to produce large amounts of IL-4. Therefore, we investigated whether basophils play an essential role in the induction of granuloma formation induced by Schistosoma mansoni eggs. Together with our previous observation that basophil numbers increased markedly in the spleen at 7 weeks postinfection, immunohistochemical staining using anti-mMCP8 monoclonal antibody (mAb) showed basophil infiltration in the granulomatous lesions formed around parasite eggs. To examine the roles of basophils more directly, we treated mice with anti-CD200R3 mAb to deplete basophils. Depletion of basophils resulted in a reduction of basophil number with concomitant downregulation of egg granuloma formation at 7 weeks postinfection. Moreover, we observed a significant reduction in the size of egg granulomas formed in basophil-depleted mice in the pulmonary granuloma model. Taken together, these findings indicated that basophils are essential for S. mansoni egg-induced granuloma formation, and this may serve as a novel therapeutic target in ameliorating the pathology of schistosomiasis. (C) 2013 Elsevier Ireland Ltd. All rights reserved.