Structure of the Arabidopsis thaliana glutamate receptor-like channel GLR3.4.
Structure of the Arabidopsis thaliana glutamate receptor-like channel GLR3.4.
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DOI：
10.1016/j.molcel.2021.05.025
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发表时间：
2021-08-05
期刊：
影响因子：
16
通讯作者：
Sobolevsky AI
中科院分区：
文献类型：
--
作者：
Green MN;Gangwar SP;Michard E;Simon AA;Portes MT;Barbosa-Caro J;Wudick MM;Lizzio MA;Klykov O;Yelshanskaya MV;Feijó JA;Sobolevsky AI
谷氨酸受体样通道（GLRs）在植物的创伤反应、气孔孔径控制、种子萌发、根系发育、先天免疫反应、花粉管生长和形态发生等生理过程中发挥着重要作用。尽管glr很重要，但对其分子结构的了解有限。本文采用x射线晶体学和单粒子低温电镜分析拟南芥GLR3.4的结构。我们的结构揭示了GLR3.4亚基的四聚体组装成三层结构域结构，让人想起动物的嗜离子性谷氨酸受体（iGluRs）。然而，GLR3.4结构域层间的非交换排列、通过氨基末端结构域内半胱氨酸C205的s -谷胱甘肽结合谷胱甘肽、独特的对称性、结构域间界面和配体特异性将GLR3.4与iGluR家族的代表分子区分开来，并表明GLR门控制机制的独特特征。我们的工作详细阐述了GLR结构和对称性的原理，并为破译植物中GLR依赖的信号机制提供了一个分子模板。Green等人研究了拟南芥谷氨酸受体样通道GLR3.4的结构，该通道显示具有三层结构的四聚体亚基组装，与其哺乳动物同源物、异离子型谷氨酸受体相似，但具有明显的对称性、结构域间界面、配体特异性和配体结合层和谷胱甘肽结合的氨基末端结构域之间的非交换结构域排列。
Glutamate receptor-like channels (GLRs) play vital roles in various physiological processes in plants, such as wound response, stomatal aperture control, seed germination, root development, innate immune response, pollen tube growth, and morphogenesis. Despite the importance of GLRs, knowledge about their molecular organization is limited. Here we employ X-ray crystallography and single-particle cryo-EM to solve structures of the Arabidopsis thaliana GLR3.4. Our structures reveal the tetrameric assembly of GLR3.4 subunits into a three-layer domain architecture, reminiscent of animal ionotropic glutamate receptors (iGluRs). However, the non-swapped arrangement between layers of GLR3.4 domains, binding of glutathione through S-glutathionylation of cysteine C205 inside the amino-terminal domain clamshell, unique symmetry, inter-domain interfaces, and ligand specificity distinguish GLR3.4 from representatives of the iGluR family and suggest distinct features of the GLR gating mechanism. Our work elaborates on the principles of GLR architecture and symmetry and provides a molecular template for deciphering GLR-dependent signaling mechanisms in plants. Green et al. solve structures of Arabidopsis thaliana glutamate receptor-like channel GLR3.4 that shows tetrameric subunit assembly with three-layer architecture, similar to its mammalian homologs, ionotropic glutamate receptors, but with distinct symmetry, inter-domain interfaces, ligand specificity and non-swapped domain arrangement between layers of ligand-binding and glutathione-bound amino-terminal domains.
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DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
16.2
作者：
Inanobe, A;Furukawa, H;Gouaux, E
通讯作者：
Gouaux, E
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
影响因子：
16.8
作者：
Burada, Ananth Prasad;Vinnakota, Rajesh;Kumar, Janesh
通讯作者：
Kumar, Janesh
影响因子：
2.2
作者：
Chen, Shaoxia;McMullan, Greg;Faruqi, Abdul R.;Murshudov, Garib N.;Short, Judith M.;Scheres, Sjors H. W.;Henderson, Richard
通讯作者：
Henderson, Richard