Genetic analysis of flag leaf size and candidate genes determination of a major QTL for flag leaf width in rice.
Genetic analysis of flag leaf size and candidate genes determination of a major QTL for flag leaf width in rice.
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DOI：
10.1186/s12284-014-0039-9
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发表时间：
2015-12
期刊：
影响因子：
--
通讯作者：
Gao Z
中科院分区：
文献类型：
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作者：
Zhang B;Ye W;Ren D;Tian P;Peng Y;Gao Y;Ruan B;Wang L;Zhang G;Guo L;Qian Q;Gao Z
旗叶是水稻进行光合作用最重要的器官，其大小在水稻理想株型育种中起着重要作用。旗叶大小在一定程度上影响光合作用，从而影响水稻产量。控制叶片大小和形状的几个基因已被鉴定为突变体。虽然在水稻不同群体的12条染色体上检测到许多叶片大小和形状的数量性状位点（qtl），但克隆的数量很少。以海南和杭州培育的两优培九重组核心自交系（LYP9）为材料，对旗叶长、宽、长宽比与单株产量进行了两两相关分析。旗叶大小和形状性状之间存在显著相关。有趣的是，旗叶宽度与单株产量呈正相关。基于我们之前构建的高分辨率连锁图谱，共检测到3个旗叶大小形状性状和单株产量的43个qtl，其中31个qtl尚未报道。在两种环境中鉴定出7个共同的qtl。在7号染色体27.1 kb区域内，定位到了一个新的旗叶宽度主QTL qFLW7.2。qFLW7.2和qPY7均位于第7染色体45.30 ~ 53.34 cM区间，与单株产量（PY）与旗叶宽度（FLW）的关系一致。qFLW7.2的93-11等位基因增加了旗叶宽度，解释了14%的表型变异。根据两个亲本之间的序列变异和表达差异筛选出两个候选基因，为进一步的超级稻QTL克隆和分子育种奠定基础。
Flag leaf is the most essential organ for photosynthesis in rice and its size plays an important role in rice breeding for ideal plant-type. Flag leaf size affect photosynthesis to a certain extent, thereby influencing rice production. Several genes controlling leaf size and shape have been identified with mutants. Although a number of quantitative trait loci (QTLs) for leaf size and shape have been detected on 12 chromosomes with different populations of rice, few of them were cloned. The pair-wise correlation analysis was conducted on length, width and length-width ratio of the flag leaf, and yield per plant in the core recombinant inbred lines of Liang-You-Pei-Jiu (LYP9) developed in Hainan and Hangzhou. There were significant correlations among the three flag leaf size and shape traits. Interestingly, a positive correlation was found between flag leaf width and yield per plant. Based on the high-resolution linkage map we constructed before, 43 QTLs were detected for three flag leaf size and shape traits and yield per plant, among which 31 QTLs were unreported so far. Seven QTLs were identified common in two environments. And qFLW7.2, a new major QTL for flag leaf width, was fine mapped within 27.1 kb region on chromosome 7. Both qFLW7.2 and qPY7 were located in the interval of 45.30 ~ 53.34 cM on chromosome 7, which coincided with the relationship between yield per plant (PY) and flag leaf width (FLW). qFLW7.2, which explained 14% of the phenotypic variation, increased flag leaf width with 93–11 allele. Two candidate genes were selected based on sequence variation and expression difference between two parents, which facilitated further QTL cloning and molecular breeding in super rice.