Effect of galacto-mannan-oligosaccharides or chitosan supplementation on cytoimmunity and humoral immunity in early-weaned piglets
Effect of galacto-mannan-oligosaccharides or chitosan supplementation on cytoimmunity and humoral immunity in early-weaned piglets
复制标题
补充半乳甘露聚糖或壳聚糖对早期断奶仔猪细胞免疫和体液免疫的影响
DOI：
10.5713/ajas.2008.70408
复制
发表时间：
2008-05-01
期刊：
影响因子：
--
通讯作者：
Kim, S. W.
中科院分区：
文献类型：
--
作者：
Yin, Y. -L.;Tang, Z. R.;Kim, S. W.
免疫调节饲料添加剂可能为养猪生产中的抗菌生长促进剂提供替代品。本试验旨在研究半乳糖甘露寡糖（GMOS）和壳寡糖（COS）对早期断奶仔猪免疫功能的影响。将平均活体重为5.6 +/- 0.51 kg的40头15日龄仔猪（Durocx Landracx Yorkshire）断奶，并随机分配至4个处理组，在2周内分别饲喂含有基础、110 mg/kg林可霉素、250 mg/kg COS或0.2% GMOS的玉米-豆粕日粮。在研究开始的同一天处死另外6头相同年龄的仔猪进行采样，以获得基线值。在15日龄和28日龄时，对每组5头猪的外周血单核细胞、空肠粘膜和淋巴结中的白细胞介素（IL）-1 β基因表达以及IL-1 β、IL-2和IL-6、伊加、IgQ和IgM的血清水平进行了评价。结果表明，断奶应激导致血清抗体和细胞因子水平下降。与补充林可霉素相比，饮食补充GMOS或COS增强（p < 0.05）空肠粘膜和淋巴结中的IL-1 β基因表达，以及IL-1 β、IL-2、IL-6、伊加、IgG和IgM的血清水平。这些结果表明，GMOS或COS可能通过调节细胞因子和抗体的产生来增强早期断奶仔猪的细胞介导的免疫应答，这表明GMOS或COS对动物生长和健康具有与抗生素不同的作用。
Immunomodulatory feed additives might offer alternatives to antimicrobial growth promoters in pig production. This experiment was designed to determine the effects of dietary gal acto-mannan-oligosaccharide (GMOS) and chitosan oligosaccharide (COS) supplementation on the immune response in early-weaned piglets. Forty 15-day-old piglets (DurocxLandracexYorkshire) with an average live body weight of 5.6 +/- 0.51 kg were weaned and randomly assigned to 4 treatment groups that were fed maize-soybean meal diets containing either basal, 110 mg/kg of lincomycin, 250 mg/kg of COS or 0.2% GMOS, respectively, over a 2-week period. Another six piglets of the same age were sacrificed on the same day at the beginning of the study for sampling, in order to obtain baseline values. Interleukin (IL)-1 beta gene expression in peripheral blood monocytes, jejunal mucosa and lymph nodes, as well as serum levels of IL-1 beta, IL-2 and IL-6, IgA, IgQ and IgM, were evaluated for 5 pigs from each group at 15 and 28 days of age. The results indicate that weaning stress resulted in decreases in serum antibody and cytokine levels. Dietary supplementation with GMOS or COS enhanced (p < 0.05) IL-1 beta gene expression in jejunal mucosa and lymph nodes, as well as serum levels of IL-1 beta, IL-2, IL-6, IgA, IgG and IgM compared to supplementation with lincomycin. These findings suggest that GMOS or COS may enhance the cell-mediated immune response in early-weaned piglets by modulating the production of cytokines and antibodies, which shows that GMOS or COS have different effects than the antibiotic on animal growth and health.