Improving lignocellulose enzymatic saccharification in a bioreactor with an applied electric field
Improving lignocellulose enzymatic saccharification in a bioreactor with an applied electric field
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DOI：
10.1016/j.indcrop.2017.08.061
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发表时间：
2017-12
影响因子：
5.9
通讯作者：
Yong-Zhong Wang;Lei Zhang;T. Xu;Ke Ding
中科院分区：
文献类型：
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作者：
Yong-Zhong Wang;Lei Zhang;T. Xu;Ke Ding
首次研制了一套脉冲电场强化木质纤维素原料酶解糖化的试验系统。采用单因素试验研究了外加电场作用下生物反应器中木质纤维素原料的酶法糖化性能。最佳工艺条件为：电场强度12 V/m，加水量5 mL/g底物，加酶量26.68 mg/g底物，pH 4.5，电极放置时间6 h。在12 V/m的外加电场强度下获得506.6mg的还原糖产率。相应的转换效率为22.8%，比无电场时提高了32.6%。结果表明，在适度的电场条件下，纤维素酶的还原糖得率和酶解糖化效率均得到提高。
A testing system with pulsed electric field was fabricated for the first time to enhance the enzymatic saccharification of lignocellulosic materials. The performance of the enzymatic saccharification of lignocellulosic material was investigated using single-factor experiment in a bioreactor with an applied electric field. The optimal conditions were identified as electric field strength of 12 V/m, supplied water content of 5 mL/g substrate, enzyme loading of 26.68 mg/g substrate, pH 4.5, electrode switchover time of 6 h. A reducing sugar yield of 506.6 mg was obtained at an applied electric field strength of 12 V/m. The corresponding conversion efficiency was 22.8%, which was 32.6% higher than that without electric field. The results show that the yield of reducing sugars and the efficiency of enzymatic saccharification of cellulose can be improved under moderate electric field conditions.