SECONDARY PLASMODESMATA ARE SPECIFIC SITES OF LOCALIZATION OF THE TOBACCO MOSAIC-VIRUS MOVEMENT PROTEIN IN TRANSGENIC TOBACCO PLANTS
SECONDARY PLASMODESMATA ARE SPECIFIC SITES OF LOCALIZATION OF THE TOBACCO MOSAIC-VIRUS MOVEMENT PROTEIN IN TRANSGENIC TOBACCO PLANTS
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DOI：
10.1105/tpc.4.8.915
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发表时间：
1992-08-01
期刊：
影响因子：
11.6
通讯作者：
LUCAS, WJ
中科院分区：
文献类型：
--
作者：
DING, B;HAUDENSHIELD, JS;LUCAS, WJ
烟草花叶病毒30-kD运动蛋白（TMV MP）基因在烟草植物中的表达增加了成熟叶片中叶肉细胞之间的胞间连丝尺寸排阻极限（SEL）10倍。在本研究中，我们研究了作为叶发育的功能的胞间连丝的结构。在30-kD TMV MP转基因（274号线）和载体对照（306号线）植物的幼叶中，几乎所有的胞间连丝都是原生的。在这两个植物系中，次生胞间连丝形成，在一个向基的模式，作为叶经历膨胀生长。超微结构和免疫标记的研究表明，在274行的TMV MP积累主要是在次生胞间连丝的非维管组织和与丝状物质。在TMV MP的存在下，检测到一个发展进程中的第四叶的尖端中的所有二级胞间连丝含有TMV MP与丝状材料。染料偶联实验表明，TMV MP诱导的胞间连丝SEL的增加可以在第四叶的顶端常规检测到，但仅限于叶肉和维管束鞘细胞。这些研究结果进行了讨论，相对于胞间连丝的结构和功能，特别是那些方面有关的病毒运动。
Expression of the tobacco mosaic virus 30-kD movement protein (TMV MP) gene in tobacco plants increases the plasmodesmatal size exclusion limit (SEL) 10-fold between mesophyll cells in mature leaves. In the present study, we examined the structure of plasmodesmata as a function of leaf development. In young leaves of 30-kD TMV MP transgenic (line 274) and vector control (line 306) plants, almost all plasmodesmata were primary in nature. In both plant lines, secondary plasmodesmata were formed, in a basipetal pattern, as the leaves underwent expansion growth. Ultrastructural and immunolabeling studies demonstrated that in line 274 the TMV MP accumulated predominantly in secondary plasmodesmata of nonvascular tissues and was associated with a filamentous material. A developmental progression was detected in terms of the presence of TMV MP; all secondary plasmodesmata in the tip of the fourth leaf contained TMV MP in association with the filamentous material. Dye-coupling experiments demonstrated that the TMV MP-induced increase in plasmodesmatal SEL could be routinely detected in the tip of the fourth leaf, but was restricted to mesophyll and bundle sheath cells. These findings are discussed with respect to the structure and function of plasmodesmata, particularly those aspects related to virus movement.