Measuring Nonspecific Protein-Protein Interactions by Dynamic Light Scattering
Measuring Nonspecific Protein-Protein Interactions by Dynamic Light Scattering
复制标题
DOI：
10.1007/978-1-4939-9678-0_1
复制
发表时间：
2019-01-01
期刊：
影响因子：
--
通讯作者：
Curtis, Robin A.
中科院分区：
文献类型：
--
作者：
Corbett, Daniel;Bye, Jordan W.;Curtis, Robin A.
动态光散射已成为测量和定量弱的非特异性蛋白质-蛋白质相互作用的一种选择方法，因为它易于使用，最小的样品消耗，并且易于通过平板阅读器进行高通量筛选。给出了如何制备蛋白质样品的程序，通过常用的实验装置进行测量，包括流过系统，板阅读器和比色皿，并分析相关函数以获得扩散系数数据。本章最后用理论部分推导并合理化了扩散系数测量与蛋白质-蛋白质相互作用之间的相关性。
Dynamic light scattering has become a method of choice for measuring and quantifying weak, nonspecific protein-protein interactions due to its ease of use, minimal sample consumption, and amenability to high-throughput screening via plate readers. A procedure is given on how to prepare protein samples, carry out measurements by commonly used experimental setups including flow through systems, plate readers, and cuvettes, and analyze the correlation functions to obtain diffusion coefficient data. The chapter concludes by a theoretical section that derives and rationalizes the correlation between diffusion coefficient measurements and protein-protein interactions.