UVR8 interacts with WRKY36 to regulate HY5 transcription and hypocotyl elongation in Arabidopsis.
UVR8 interacts with WRKY36 to regulate HY5 transcription and hypocotyl elongation in Arabidopsis.
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UVR8 与 WRKY36 相互作用，调节拟南芥中 HY5 转录和下胚轴伸长。
DOI：
10.1038/s41477-017-0099-0
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Liu Hongtao
中科院分区：
文献类型：
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作者：
Yang Yu;Liang Tong;Zhang Libo;Shao Kai;Gu Xingxing;Shang Ruixin;Shi Nan;Li Xu;Zhang Peng;Liu Hongtao
UV抗性基因座8（UV Resistance Locus 8，UVR 8）是植物中介导光响应的紫外线-B（UVB）辐射感光体。植物UVR 8如何响应UVB光还不清楚。在这里，我们报告的鉴定和表征的tenopsis WRKY DNA结合蛋白36（WRKY 36）蛋白。WRKY 36在酵母和拟南芥细胞中与UVR 8相互作用，通过抑制HY 5的转录促进下胚轴的伸长。在UVB下抑制下胚轴伸长需要抑制WRKY 36。WRKY 36与HY 5启动子的W-box基序结合抑制其转录，而核定位的UVR 8直接与WRKY 36相互作用抑制WRKY 36-DNA结合，导致释放对HY 5转录的抑制。这些结果表明，WRKY 36是HY 5的负调控因子，UVB通过UVR 8抑制WRKY 36，从而促进HY 5的转录和光形态建成。UVR 8-WRKY 36在细胞核中的相互作用代表了拟南芥早期UVR 8信号转导的一种新机制。
UV RESISTANCE LOCUS 8 (UVR8) is an ultraviolet-B (UVB) radiation photoreceptor that mediates light responses in plants. How plant UVR8 acts in response to UVB light is not well understood. Here, we report the identification and characterization of theArabidopsisWRKY DNA-BINDING PROTEIN 36 (WRKY36) protein. WRKY36 interacts with UVR8 in yeast andArabidopsiscells and it promotes hypocotyl elongation by inhibitingHY5transcription. Inhibition of hypocotyl elongation under UVB requires the inhibition of WRKY36. WRKY36 binds to the W-box motif of theHY5promoter to inhibit its transcription, while nuclear localized UVR8 directly interacts with WRKY36 to inhibit WRKY36–DNA binding both in vitro and in vivo, leading to the release of inhibition ofHY5transcription. These results indicate that WRKY36 is a negative regulator ofHY5and that UVB represses WRKY36 via UVR8 to promote the transcription ofHY5and photomorphogenesis. The UVR8–WRKY36 interaction in the nucleus represents a novel mechanism of early UVR8 signal transduction inArabidopsis.