Effect of 2-methoxyestradiol, buthionine sulfoximine and hydrogen peroxide on the viability of renal carcinoma cell lines (ACHN and ACVB)
Effect of 2-methoxyestradiol, buthionine sulfoximine and hydrogen peroxide on the viability of renal carcinoma cell lines (ACHN and ACVB)
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DOI：
10.1248/bpb.29.1064
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发表时间：
2006-05-01
影响因子：
2
通讯作者：
Iwakawa, Seigo
中科院分区：
文献类型：
--
作者：
Hirai, Yuka;Kawabe, Noriko;Iwakawa, Seigo
2-甲氧基雌二醇(2-ME)是17-β-雌二醇的内源性代谢产物，可诱导细胞内超氧阴离子(O-2(-))的积累，而丁硫氨酸亚磺胺(BSO)是谷胱甘肽(GSH)合成的抑制剂。我们考察了2-ME和BSO与过氧化氢(H_2O_2)的联合抗癌作用。2-ME抑制肾癌细胞株(ACHN和ACVB)的生长，并伴随细胞内GSH含量的增加。2-ME、BSO和H_2O_2联合使用对ACHN和ACVB均有明显的抑制增殖作用。加入2-ME和H_2O_2后，BSO对ACHN和ACVB细胞内ROS的影响显著，这可能是其抗增殖作用的机制之一。
2-Methoxyestradiol (2-ME), an endogenous metabolite of 17 beta-estradiol, induces the intracellular accumulation of superoxide anion (O-2(-)) and buthionine sulfoximine (BSO) is an inhibitor of glutathione (GSH) synthesis. We have examined the combination anticancer effect of 2-ME and BSO accompanied with hydrogen peroxide (H2O2). 2-ME inhibited cell growth in renal carcinoma cell lines (ACHN and ACVB) accompanied by an increase in the intracellular contents of GSH. The combination of 2-ME, BSO and H2O2 showed a significant antiproliferation effect in both ACHN and ACVB. The intracellular levels of reactive oxygen species (ROS) with a combination with 2-ME and H2O2 in ACHN and ACVB pretreated with BSO were markedly increased, which may have contributed to the potential antiproliferative action.