Laminin in lung development: effects of anti-laminin antibody in murine lung morphogenesis.
Laminin in lung development: effects of anti-laminin antibody in murine lung morphogenesis.
复制标题
肺发育中的层粘连蛋白：抗层粘连蛋白抗体对小鼠肺形态发生的影响。
DOI：
10.1016/0012-1606(90)90004-3
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发表时间：
1990
影响因子：
2.7
通讯作者：
Varani,J
中科院分区：
文献类型：
--
作者：
Schuger,L;O'Shea,S;Rheinheimer,J;Varani,J
研究了胎鼠肺形态发生过程中基底膜主要糖蛋白层粘连蛋白的沉积模式和作用。从第13天胚胎中取出肺原基，进一步解剖右下叶并置于过滤膜组件中。然后将外植体在液-气界面中培养3天，在抗层粘连蛋白、抗血栓反应蛋白（另一种细胞外基质成分）、免疫前血清、层粘连蛋白中和的抗层粘连蛋白或单独培养基中培养。用（直接）相差显微镜、光学显微镜和免疫荧光法监测培养物。我们发现抗层粘连蛋白抗体以剂量依赖的方式改变正常的肺形态发生。抗层粘连蛋白处理的外植体表现出明显的分支形态发生抑制和支气管树畸变。暴露于该抗体的外植体也观察到较低的生长速率。高浓度的抗血栓反应蛋白抗体、正常兔血清或层粘连蛋白中和的抗层粘连蛋白抗体对肺形态发生没有影响。这些结果不改变外植体在浸没培养或在Vitrogen 100包被表面上的培养。
The pattern of deposition and the role of laminin, a major glycoprotein constituent of basement membranes, were investigated during lung morphogenesis in the fetal mouse. Lung primordia were removed from Day 13 embryos, right lower lobes were further dissected and placed in filter membrane assemblies. Explants were then cultured at the liquid-air interface for 3 days in the presence of anti-laminin, anti-thrombospondin (another extracellular matrix constituent), preimmune serum, laminin-neutralized anti-laminin, or medium alone. Cultures were monitored by (direct) phase-contrast microscopy, light microscopy, and immunofluorescence. We found that anti-laminin antibodies altered normal lung morphogenesis in a dose-dependent manner. The anti-laminin-treated explants presented a marked inhibition of branching morphogenesis and a distortion of the bronchial tree. A lower rate of growth was also observed in the explants exposed to this antibody. High concentrations of anti-thrombospondin antibodies, normal rabbit serum, or laminin-neutralized anti-laminin antibodies had no effect on lung morphogenesis. These results were not modified by culturing the explants in submersion culture or on Vitrogen 100-coated surfaces.