Stimulation of Mammalian G-protein-responsive Adenylyl Cyclases by Carbon Dioxide
Stimulation of Mammalian G-protein-responsive Adenylyl Cyclases by Carbon Dioxide
复制标题
二氧化碳对哺乳动物 G 蛋白反应性腺苷酸环化酶的刺激
DOI：
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发表时间：
2009
影响因子：
4.8
通讯作者：
M. Cann
中科院分区：
文献类型：
--
作者：
Philip D. Townsend;Phillip M. Holliday;Stepan Fenyk;K. Hess;M. Gray;David R. W. Hodgson;M. Cann
Carbon dioxide is fundamental to the physiology of all organisms. There is considerable interest in the precise molecular mechanisms that organisms use to directly sense CO2. Here we demonstrate that a mammalian recombinant G-protein-activated adenylyl cyclase and the related Rv1625c adenylyl cyclase of Mycobacterium tuberculosis are specifically stimulated by CO2. Stimulation occurred at physiological concentrations of CO2 through increased kcat. CO2 increased the affinity of enzyme for metal co-factor, but contact with metal was not necessary as CO2 interacted directly with apoenzyme. CO2 stimulated the activity of both G-protein-regulated adenylyl cyclases and Rv1625c in vivo. Activation of G-protein regulated adenylyl cyclases by CO2 gave a corresponding increase in cAMP-response element-binding protein (CREB) phosphorylation. Comparison of the responses of the G-protein regulated adenylyl cyclases and the molecularly, and biochemically distinct mammalian soluble adenylyl cyclase revealed that whereas G-protein-regulated enzymes are responsive to CO2, the soluble adenylyl cyclase is responsive to both CO2 and bicarbonate ion. We have, thus, identified a signaling enzyme by which eukaryotes can directly detect and respond to fluctuating CO2.
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DOI：
10.1073/pnas.0400050101
发表时间：
2004-03-02
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
2.7
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影响因子：
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作者：
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影响因子：
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