Vibration enhances osteoclastogenesis by inducing RANKL expression via NF-κB signaling in osteocytes
Vibration enhances osteoclastogenesis by inducing RANKL expression via NF-κB signaling in osteocytes
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DOI：
10.1016/j.bone.2019.03.024
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发表时间：
2019-06-01
期刊：
影响因子：
4.1
通讯作者：
Takano-Yamamoto, Teruko
中科院分区：
文献类型：
--
作者：
Sakamoto, Mayuri;Fukunaga, Tomohiro;Takano-Yamamoto, Teruko
为了缩短正畸治疗的持续时间，不仅要减少龋齿、牙周病和牙根吸收等风险，而且要减少固定矫治器引起的疼痛和不适。有几项研究调查了施加到固定矫治器上的振动对加速牙齿移动的影响。虽然有报道振动通过促进牙槽骨吸收加速正畸牙齿移动，但其潜在的细胞和分子机制尚不清楚。在这项研究中，我们研究了振动对破骨细胞在体外和体内的影响。施加到破骨细胞前细胞系RAW 264.7细胞的振动促进细胞增殖，但不影响它们向破骨细胞的分化。已知骨中的骨细胞是机械敏感的，并且充当核因子κ B（NF-κ B）配体（RANKL）的受体激活剂。因此，在本研究中，振动施加到来自骨细胞样细胞系MLO-Y 4的细胞。在MLO-Y 4细胞中，振动诱导NF-κ B抑制剂（I κ B）的磷酸化，并导致NF-κ B p65的核定位。此外，振动增加RANKL mRNA的表达，但不影响骨保护素（OPG）mRNA的表达在MLO-Y 4细胞，从而导致增加RANKL/OPG的比例。与这些发现一致，在实验性牙齿移动过程中施加的振动增加了体内牙槽骨压缩侧骨细胞中NF-κ B活化和RANKL表达，而振动对张力侧没有这种影响。此外，在MLO-Y 4细胞和RAW 264.7细胞的共培养物中，施加到MLO-Y 4细胞的振动增强破骨细胞生成。这些结果表明，振动可以通过增加前破骨细胞的数量和通过激活NF-κ B B上调骨细胞中RANKL的表达来促进破骨细胞的生成，从而加速正畸牙齿的移动。
To shorten the duration of orthodontic treatment it is important not only to reduce risks such as dental caries, periodontal disease, and root resorption, but also to decrease pain and discomfort caused by a fixed appliance. Several studies have investigated the effect of vibration applied to fixed appliances to accelerate tooth movement. Although it was reported that vibration accelerates orthodontic tooth movement by enhancing alveolar bone resorption, the underlying cellular and molecular mechanisms remain unclear. In this study, we investigated the effects of vibration on osteoclastogenesis in vitro and in vivo. Vibration applied to pre-osteoclast cell line RAW264.7 cells enhanced cell proliferation but did not affect their differentiation into osteoclasts. Osteocytes in bone are known to be mechanosensitive and to act as receptor activator of nuclear factor kappa B (NF-kappa B) ligand (RANKL). Therefore, in the present study, vibration was applied to cells from the osteocyte-like cell line MLO-Y4. In MLO-Y4 cells, vibration induced phosphorylation of the inhibitor of NF-kappa B (I kappa B) and caused nuclear localization of NF-kappa B p65. Additionally, vibration increased RANKL mRNA expression, but did not affect osteoprotegerin (OPG) mRNA expression in MLO-Y4 cells, thus resulting in an increased RANKL/OPG ratio. Consistent with these findings, vibration applied during experimental tooth movement increased NF-kappa B activation and RANKL expression in osteocytes on the compression side of alveolar bone in vivo, whereas vibration had no such effects on the tension side. Furthermore, in a co-culture of MLO-Y4 cells and RAW264.7 cells, vibration applied to MLO-Y4 cells enhanced osteoclastogenesis. These findings suggest that vibration could accelerate orthodontic tooth movement by enhancing osteoclastogenesis through increasing the number of pre-osteoclasts and up-regulating RANKL expression in osteocytes on the compression side of alveolar bone via NF-kappa B activation.