A tail of two phages: genomic and functional analysis of Listeria monocytogenes phages vB_LmoS_188 and vB_LmoS_293 reveal the receptor-binding proteins involved in host specificity.
A tail of two phages: genomic and functional analysis of Listeria monocytogenes phages vB_LmoS_188 and vB_LmoS_293 reveal the receptor-binding proteins involved in host specificity.
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DOI：
10.3389/fmicb.2015.01107
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发表时间：
2015
影响因子：
5.2
通讯作者：
McAuliffe O
中科院分区：
文献类型：
--
作者：
Casey A;Jordan K;Neve H;Coffey A;McAuliffe O
噬菌体的物理特性使其成为病原体检测分析和生物防治措施下游开发的可行候选者。要利用噬菌体达到这一目的，对其宿主相互作用机制的详细了解是先决条件。目前关于革兰氏阴性噬菌体-宿主相互作用的知识很丰富，但与革兰氏阳性噬菌体和李斯特氏菌噬菌体的相互作用相比却很少。本研究测定了最近分离的两株单核细胞增生性李斯特菌噬菌体(VB_LMOS_188和VB_LMOS_293)的裂解谱，并用比较基因组学方法研究了它们观察到的4b/4E型宿主特异性的基因组基础。这些噬菌体的晚期尾部基因被鉴定为与其他血清型4特异性李斯特菌噬菌体相比高度保守。这些噬菌体中的每一个都产生了具有扩大宿主特异性的自发突变。将它们的尾部基因序列与野生型进行比较，结果表明VB_LMOS_188的ORF 19和VB_LMOS_293的ORF 20的产物可能是这些噬菌体的受体结合蛋白。研究结果还表明，具有不同宿主特异性的李斯特菌噬菌体存在保守的底板结构和宿主相互作用机制，这进一步有助于目前关于李斯特菌噬菌体与宿主相互作用的知识。
The physical characteristics of bacteriophages establish them as viable candidates for downstream development of pathogen detection assays and biocontrol measures. To utilize phages for such purposes, a detailed knowledge of their host interaction mechanisms is a prerequisite. There is currently a wealth of knowledge available concerning Gram-negative phage-host interaction, but little by comparison for Gram-positive phages and Listeria phages in particular. In this research, the lytic spectrum of two recently isolated Listeria monocytogenes phages (vB_LmoS_188 and vB_LmoS_293) was determined, and the genomic basis for their observed serotype 4b/4e host-specificity was investigated using comparative genomics. The late tail genes of these phages were identified to be highly conserved when compared to other serovar 4-specific Listeria phages. Spontaneous mutants of each of these phages with broadened host specificities were generated. Their late tail gene sequences were compared with their wild-type counterparts resulting in the putative identification of the products of ORF 19 of vB_LmoS_188 and ORF 20 of vB_LmoS_293 as the receptor binding proteins of these phages. The research findings also indicate that conserved baseplate architectures and host interaction mechanisms exist for Listeria siphoviruses with differing host-specificities, and further contribute to the current knowledge of phage-host interactions with regard to Listeria phages.