Photopolymerized Features via Beam Pen Lithography as a Novel Tool for the Generation of Large Area Protein Micropatterns
Photopolymerized Features via Beam Pen Lithography as a Novel Tool for the Generation of Large Area Protein Micropatterns
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通过束笔光刻的光聚合特征作为生成大面积蛋白质微图案的新工具
DOI：
10.1002/smll.202105998
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发表时间：
2022
期刊：
影响因子：
13.3
通讯作者：
Mirkin, Chad A.
中科院分区：
文献类型：
--
作者：
Zhang, Xinpeng;Ding, Shaowei;Magoline, Jared;Ivankin, Andrey;Mirkin, Chad A.
描述了一种基于无悬臂扫描探针光刻(CF-SPL)的方法，通过交联和硫醇-丙烯酸酯光反应在表面上快速聚合纳米级特征，其中每个特征的纳米级位置、高度和直径可以精细而独立地调节。通过对照明图案的精确时空控制，光束笔光刻(BPL)允许使用大规模平行的可单独寻址的笔阵列>；160 000笔阵列，以亚衍射分辨率引导光并将光聚焦到表面上，从而将聚合物光交联成厘米级区域的超高分辨率特征。该油墨材料由光引发剂、二苯基(2，4，6-三甲基苯甲酰基)氧膦、聚乙二醇二丙烯酸酯和硫醇修饰的功能结合分子(即硫醇-聚乙二醇生物素或16-巯基己酸)组成，在短时间(0.5%S)的紫外光照射下(72 mW/cm≈2)进行80%的转化为−。这种聚合物图案进一步与蛋白质(链霉亲和素和纤维连接蛋白)反应，产生具有高分辨率和由局部紫外线照射控制的密度的特征排列的蛋白质阵列。该平台结合了聚合物光化学和大量扫描探针阵列，构成了高通量和高产率制造生物分子微阵列的新途径，为生物芯片合成、生物筛选和细胞生物学研究开辟了新的途径。
A cantilever‐free scanning probe lithography (CF‐SPL)‐based method for the rapid polymerization of nanoscale features on a surface via crosslinking and thiol‐acrylate photoreactions is described, wherein the nanoscale position, height, and diameter of each feature can be finely and independently tuned. With precise spatiotemporal control over the illumination pattern, beam pen lithography (BPL) allows for the photo‐crosslinking of polymers into ultrahigh resolution features over centimeter‐scale areas using massively parallel >160 000 pen arrays of individually addressable pens that guide and focus light onto the surface with sub‐diffraction resolution. The photoinduced crosslinking reaction of the ink material, which is composed of photoinitiator, diphenyl(2,4,6‐trimethylbenzoyl) phosphine oxide, poly(ethylene glycol) diacrylate, and thiol‐modified functional binding molecules (i.e., thiol‐PEG‐biotin or 16‐mercaptohexanoic acid), proceeds to ≈80% conversion with UV exposure (72 mW cm−2) for short time periods (0.5 s). Such polymer patterns are further reacted with proteins (streptavidin and fibronectin) to yield protein arrays with feature arrangements at high resolution and densities controlled by local UV exposure. This platform, which combines polymer photochemistry and massive arrays of scanning probes, constitutes a new approach to making biomolecular microarrays in a high‐throughput and high‐yielding manner, opening new routes for biochip synthesis, bioscreening, and cell biology research.