Disruption of nodal architecture in skin biopsies of patients with demyelinating neuropathies
Disruption of nodal architecture in skin biopsies of patients with demyelinating neuropathies
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DOI：
10.1111/jns5.12023
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发表时间：
2013-06-01
影响因子：
3.8
通讯作者：
Sommer, Claudia
中科院分区：
文献类型：
--
作者：
Doppler, Kathrin;Werner, Christian;Sommer, Claudia
皮神经是感觉神经系统的最末端。我们利用皮肤活检中纵向髓鞘纤维的良好可辨别性来分析神经病变中淋巴结和副淋巴结蛋白的分布，并评估淋巴结紊乱作为脱髓鞘神经病变（NP）的诊断标志。我们分析了52例不同周围神经病变患者和17例对照组的手指和腿近端皮肤活检中的髓鞘神经纤维。我们用抗mbp、抗pgp9.5、抗caspr、抗泛神经束蛋白和抗泛钠通道进行免疫组化双标记。脱髓鞘NP的三个潜在特征可以确定：脱髓鞘比轴突神经性病变更常发现朗维耶淋巴结延长和接触蛋白相关蛋白（caspr）染色分散(p
Cutaneous nerves represent the most distal part of the sensory nervous system. We took advantage of the good discernibility of longitudinal myelinated fibers in skin biopsies to analyze the distribution of nodal and paranodal proteins in neuropathies and to assess nodal disorganization as a diagnostic marker of demyelinating neuropathy (NP). We analyzed myelinated nerve fibers in skin biopsies from the finger and the proximal leg of 52 prospectively recruited patients with different peripheral neuropathies and 17 controls. We performed immunohistochemical double labeling with anti-MBP, anti-PGP9.5, anti-caspr, anti-pan-neurofascin, and anti-pan-sodium-channel. Three potential features of demyelinating NP could be established: elongated nodes of Ranvier and dispersion of contactin-associated protein (caspr) staining were found more often in demyelinating than in axonal neuropathies (p