Simultaneous Measurement of Surface Proteins and Gene Expression from Single Cells.
Simultaneous Measurement of Surface Proteins and Gene Expression from Single Cells.
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同时测量单细胞的表面蛋白和基因表达。
DOI：
10.1007/978-1-0716-0266-9_3
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Chen,Kong
中科院分区：
文献类型：
--
作者：
Luo,Jiadi;Erb,CarlaA;Chen,Kong
单细胞转录组学分析已成为研究复杂多细胞系统的一种新的有力工具。单细胞RNA测序在转录水平上提供了对异质细胞状态的公正分类，但它并不总是与细胞表面蛋白表达相关。最近开发的一种称为通过测序的转录组和表位的细胞索引（CITE-seq）的方法同时测量单细胞的表面蛋白和基因表达。简单地说，基于现有的单细胞测序技术，使用多核苷酸标记的抗体和条形码化的引物凝胶珠分别与相应的细胞表面蛋白和mRNA结合。此外，对标记的蛋白质和RNA信息的文库进行测序以将细胞蛋白质和转录组读数有效地整合在一起。CITE-seq正在将全面的基因组研究转变为因果基因-蛋白质研究的模型。
Single-cell transcriptomic analysis has become a new and powerful tool to study complex multicellular systems. Single-cell RNA sequencing provides an unbiased classification of heterogeneous cellular states at the transcriptional level, but it does not always correlate to cell-surface protein expression. A recently developed method called cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) simultaneously measures surface proteins and gene expression from single cells. Briefly, based on the existing single-cell sequencing technology, oligonucleotide-labeled antibodies and barcoded primer gel beads are used to bind to corresponding cell-surface proteins and mRNA, respectively. Further, libraries of labeled protein and RNA information are sequenced to integrate cellular protein and transcriptome reads together efficiently. CITE-seq is transforming comprehensive genomic studies into models of causal gene-protein investigation.