Characterization of the ETnII-alpha endogenous retroviral element in the BALB/cJ Zhx2 ( Afr1 ) allele.
Characterization of the ETnII-alpha endogenous retroviral element in the BALB/cJ Zhx2 ( Afr1 ) allele.
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BALB/cJ Zhx2 ( Afr1 ) 等位基因中 ETnII-α 内源性逆转录病毒元件的表征。
DOI：
10.1007/s00335-007-9077-6
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
Spear,BrettT
中科院分区：
文献类型：
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作者：
Perincheri,Sudhir;Peyton,DavidK;Glenn,Michelle;Peterson,MarthaL;Spear,BrettT
小鼠内源性逆转录病毒（MERV）元素的整合导致了实验室小鼠中大约10%的自发突变。我们最近在BALB/cJ小鼠的锌指和同源盒2（Zhx2）基因的第一个内含子中发现了MERV整合，导致Zhx2表达降低。这种整合在BALB/cJ中发现，但在其他BALB/c亚株中没有发现，表明它发生在这些亚株于20世纪30年代末分离之后。我们对这个MERV元素进行了表征，并表明它属于etni -α类元素。我们的分析表明，与大多数其他ETn元件相比，zhx2etn元件缺少69-bp的序列，这可能是由于两个相同的13-bp元件之间的重组。在BALB/cJ小鼠的肝脏中发现了三个成熟的zhx2转录本。主要转录本从zhx2exon 1拼接到5 ' ETn LTR，并在3 ' LTR处进行聚adenylation。在两个较少的转录本中，一个与野生型转录本相同，而第二个转录本包含183 bp的ETn序列拼接在zhx2exon 1和2之间。我们还对MRL/lpr小鼠中发现的faslpretn的产物进行了测序和分析，表明它属于etni -β类元件。
Integration of mouse endogenous retroviral (MERV) elements is responsible for an estimated 10% of spontaneous mutations that have been characterized in the laboratory mouse. We recently identified a MERV integration in the first intron of theZinc fingers and homeoboxes 2(Zhx2) gene in BALB/cJ mice, resulting in reducedZhx2expression. This integration is found in BALB/cJ but not in other BALB/c substrains, indicating that it occurred after these substrains separated in the late 1930s. We have characterized this MERV element and show here that it belongs to the ETnII-α class of elements. Our analysis reveals that theZhx2ETn element lacks a 69-bp sequence compared to most other ETn elements which may be due to recombination between two identical 13-bp elements. Three matureZhx2transcripts are found in the liver of BALB/cJ mice. The major transcript is spliced fromZhx2exon 1 to the 5′ ETn LTR and is polyadenylated at the 3′ LTR. Of the two less abundant transcripts, one is identical to the wild-type transcript, whereas the second contains 183 bp of ETn sequence spliced betweenZhx2exons 1 and 2. We have also sequenced and analyzed products from thefaslprETn found in MRL/lpr mice and show that it belongs to the ETnII-β class of elements.