Evaluation of the efficacy of real-time polymerase chain reaction for the routine early detection of Pseudomonas aeruginosa in cystic fibrosis sputum and throat swab specimens
Evaluation of the efficacy of real-time polymerase chain reaction for the routine early detection of Pseudomonas aeruginosa in cystic fibrosis sputum and throat swab specimens
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DOI：
10.1016/j.diagmicrobio.2010.07.012
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发表时间：
2010-12-01
影响因子：
2.9
通讯作者：
O'Sullivan, Niamh
中科院分区：
文献类型：
--
作者：
Logan, Catriona;Habington, Adele;O'Sullivan, Niamh
对 183 名囊性纤维化儿童患者在 29 个月内采集的 2099 份痰液和咽拭子进行的纵向研究，用于评估实时聚合酶链式反应 (PCR) 与微生物培养相比，早期检测铜绿假单胞菌的效果。实时 PCR 导致被鉴定为铜绿假单胞菌阳性的标本数量增加。培养的敏感性为 82% (373/453) 和当考虑阳性培养和 PCR 结果为真阳性时，PCR 为 93% (420/453) 在被鉴定为铜绿假单胞菌 PCR 阳性/培养阴性的 80 份标本中，随后的患者样本中有 32 5% (26/80) 相关标本被鉴定为铜绿假单胞菌培养阳性，这表明 PCR 有可能比微生物培养物更早检测出铜绿假单胞菌 实时PCR 分析未发现所检查队列中利物浦和曼彻斯特流行铜绿藻菌株的证据 这项研究的结果强调了样本采集方案的重要性，以确保实验室收到足够的样本进行测试，从而最大限度地减少报告假阴性铜绿藻培养结果的可能性 (C) 2010 Elsevier Inc 保留所有权利
A longitudinal study of 2099 sputa and throat swabs received from 183 pediatric cystic fibrosis patients over a 29-month period was used to evaluate the efficacy of real-time polymerase chain reaction (PCR) for the early detection of Pseudomonas aeruginosa as compared to microbiologic culture Real-time PCR resulted in an increased number of specimens identified as P aerugmosa positive The sensitivity of culture was 82% (373/453) and of PCR was 93% (420/453) when considering both positive culture and PCR results as true positives Of the 80 specimens identified as PCR positive/culture negative for P aerugmosa, the subsequent patient sample in 32 5% (26/80) of specimens concerned was identified as P aerugmosa culture positive, suggesting that PCR has the potential to detect P aeruginosa earlier than the microbiologic culture Real-time PCR analysis found no evidence of the Liverpool and Manchester epidemic P aerugmosa strains in the cohort examined The findings of this study highlight the importance of specimen collection protocols to ensure that adequate samples are received at the laboratory for testing, thereby minimizing the potential for reporting of false-negative P aerugmosa culture results (C) 2010 Elsevier Inc All rights reserved