THE EFFECT OF MERCURIC-SALTS ON THE ELECTROROTATION OF YEAST-CELLS AND COMPARISON WITH A THEORETICAL-MODEL
THE EFFECT OF MERCURIC-SALTS ON THE ELECTROROTATION OF YEAST-CELLS AND COMPARISON WITH A THEORETICAL-MODEL
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DOI：
10.1016/0005-2736(87)90276-8
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发表时间：
1987-06-12
期刊：
影响因子：
--
通讯作者：
ZIMMERMANN, U
中科院分区：
文献类型：
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作者：
GEIER, BM;WENDT, B;ZIMMERMANN, U
酵母细胞的旋转光谱改变后的细胞与HgCl 2或HgCl 2或Hg（NO3）的预处理，并成为无法区分的超声波产生的细胞壁。受影响细胞的光谱包含一个峰值，这只能通过将电导率归因于高于培养基的细胞壁来解释。转动响应的理论模型与实验谱完全符合雅阁。结果表明，旋转方法能够测量甚至低的酵母细胞的细胞壁电导率（发现在10 μ S/cm培养基电导率下为33 μ S/cm）。知识的光谱允许一个字段的频率被选择在未处理的细胞显示没有旋转，但在该细胞受影响的治疗与汞（II）确定自己的旋转方向相同的领域。计算显示这种共场旋转的细胞的百分比，得到受影响的细胞比例的指数（称为共场旋转值）。使用这种技术，25纳摩尔/升汞（II）的影响，可以证明。在低电导率（10 μ S/cm）的介质中，转动光谱的变化通常是“全或无”，而在200 μ S/cm时，分级的Hg（II）介导的变化变得明显。共场旋转法表明，少量的Hg（II）的作用仍然是增加后，3小时的孵育和抑制Hg（II）诱导的K+释放。当3-30 mM K+（或Na+）或1 mM Ca 2+存在于培养介质中时，或当pH值增加时，可以看到Hg（II）的影响迅速减少。在高孵化细胞浓度的汞（II）的毒性作用减少，显然是由于结合的细胞。
The rotational spectrum of yeast cells changed after pre-treatment of the cells with HgCl2 or HgCl2 or Hg(NO3) and became indistinguishable from that of ultrasonically produced cell walls. The spectum of the affected cells contained a peak which could only be explained by attributing a conductivity to the cell walls that was higher than that of the medium. Theoretical models of the rotational response are fully in accord with the experimental spectra. It is shown that the rotation method is capable of measuring even the low cell wall conductivity of yeast cells (which was found to be 33 .mu.S/cm at 10 .mu.S/cm medium conductivity). Knowledge of the spectra allowed a field frequency to be selected at which untreated cells showed no rotation, but at which cells affected by treatment with Hg(II) identified themselves by rotating in the same direction as the field. Calculation of the percentage of cells showing this co-field rotation gave an index (termed the co-field rotation value) of the proportion of the cells that were affected. Using this technique, effects of 25 nmol/1 Hg(II) could be demonstrated. In media of low conductivity (10 .mu.S/cm) the change in the rotational spectrum usually ''all-or-none'', whereas at 200 .mu.S/cm a graded Hg(II)-mediated change became apparent. The co-field rotation method showed that the action of small quantities of Hg(II) was still increasing after 3 h of incubation and paralleled the Hg(II)-induced K+ release. A rapid reduction of the effects of Hg(II) was seen when 3-30 mM K+ (or Na+) or when 1 mM Ca2+ were present in the incubation medium, or as the pH was increased. At high incubation cell concentrations the toxic effect of Hg(II) was reduced, apparently due to binding by the cells.