Secondary structure of the autoregulatory mRNA binding site of ribosomal protein L1.
Secondary structure of the autoregulatory mRNA binding site of ribosomal protein L1.
复制标题
核糖体蛋白 L1 的自动调节 mRNA 结合位点的二级结构。
DOI：
10.1007/bf00337759
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Nomura,M
中科院分区：
文献类型：
--
作者：
Kearney,KR;Nomura,M
用酶法研究了大肠杆菌核糖体蛋白L1自身调节mRNA结合位点的二级结构。E.将coliL11操纵子克隆到沙门氏菌噬菌体SP6启动子控制下的载体中，并使用SP6 RNA聚合酶转录RNA。使用结构特异性核酸酶探测这种RNA的二级结构，并通过与计算机预测的RNA折叠的数据进行比较，推断出二级结构特征。所提出的模型是一致的一些先前提出的模型的元素，但在其他功能不同。最后，从两个突变体mRNA中获得二级结构信息，并且结构特征与突变体的观察表型相关。
The secondary structure of the autoregulatory mRNA binding site ofEscherichia coliribosomal protein L1 has been studies using enzymatic methods. The control region of theE. coliL11 operon was cloned into a vector under control of theSalmonellaphage SP6 promoter, and RNA transcribed using SP6 RNA polymerase. The secondary structure of this RNA was probed using structure-specific nucleases, and by comparison of the data with computer predictions of RNA folding, secondary structural features were deduced. The proposed model is consistent with elements of some previously proposed models, but differs in other features. Finally, secondary structure information was obtained from two mutant mRNAs and the structural features correlated with observed phenotypes of the mutants.