Stimulation of cyclic AMP, 17 beta-oestradiol and protein synthesis by human chorionic gonadotrophin in human endometrial cells
Stimulation of cyclic AMP, 17 beta-oestradiol and protein synthesis by human chorionic gonadotrophin in human endometrial cells
复制标题
DOI：
10.1093/humrep/12.9.1903
复制
发表时间：
1997-09-01
影响因子：
6.1
通讯作者：
Bhattacharya, S
中科院分区：
文献类型：
--
作者：
Chatterjee, A;Jana, NR;Bhattacharya, S
从 30-37 岁排卵女性中收集人子宫内膜组织，并进行酶分散和处理以获得子宫内膜基质细胞。细胞（2x10(4)/孔）在不存在（对照）或存在人绒毛膜促性腺激素（HCG）（10 IU/孔）的情况下体外孵育 1 小时（测定 cAMP）或 4 小时（估计蛋白质合成，17 β-雌二醇和芳香酶活性），与对照相比，HCG显着刺激细胞cAMP含量和释放，在没有磷酸二酯酶（PDE）抑制剂的情况下，细胞cAMP的积累和释放在10分钟时达到峰值，然后急剧下降，或者在存在PDE抑制剂的情况下保持稳定1小时，基质细胞中17β-雌二醇的含量和释放从30分钟到1小时急剧增加，然后在响应的4小时内缓慢增加与 HCG 相比，与对照相比，用 HCG 孵育细胞显着刺激 (P < 0.01) 细胞芳香酶活性，HCG 分两个阶段刺激细胞的蛋白质合成，最初在 15 分钟时保持稳定 1 小时，然后在 1 至 2 小时之间显着增加 (P < 0.01)。蛋白质合成的初始阶段似乎是 HCG 的直接影响，而后期则是由于 HCG 引起的雌二醇浓度较高，蛋白质合成的初始阶段受到放线菌酮抑制，但不被放线菌素 D 抑制，而蛋白质合成的后期阶段则受到放线菌酮和放线菌素 D 的抑制，因此结果表明 HCG 对基质细胞作用的可能机制，并证明了人类促性腺激素受体的生理相关性该实验室早些时候报道的子宫内膜细胞。
Human endometrial tissue was collected from 30-37 year old ovulatory women and enzymatically dispersed and processed to obtain endometrial stromal cells, Cells (2x10(4)/well) were incubated in vitro in the absence (control) or presence of human chorionic gonadotrophin (HCG) (10 IU/well) either for 1 h (to determine cAMP) or for 4 h (to estimate protein synthesis, 17 beta-oestradiol and aromatase activity), HCG significantly stimulated cellular cAMP content and release in comparison to control, Both cellular accumulation and release of cAMP reached the peak at 10 min and then sharply declined in the absence of phosphodiesterase (PDE) inhibitor or remained steady for 1 h in the presence of PDE inhibitor, Content and release of 17 beta-oestradiol from stromal cells steeply increased from 30 min to 1 h and then slowly increased for 4 h in response to HCG, Incubation of cells with HCG significantly stimulated (P < 0.01) cellular aromatase activity in comparison to control, HCG stimulated protein synthesis of the cells in two phases, initially at 15 min which remained steady for 1 h and then significantly increased (P < 0.01) between 1 and 2 h. The initial phase of protein synthesis appears to be the direct effect of HCG while the later phase was due to the higher concentration of oestradiol caused by HCG, The initial phase of protein synthesis was inhibited by cycloheximide but not by actinomycin D, whereas the later phase of protein synthesis was inhibited both by cycloheximide and actinomycin D, Results therefore indicate a probable mechanism of HCG action on stromal cells and demonstrate the physiological relevance of gonadotrophin receptor in human endometrial cells reported earlier from this laboratory.