ASSESSMENT OF BEAUVERIA-BASSIANA NOV EO-1 STRAIN, WHICH PRODUCES A RED PIGMENT FOR MICROBIAL CONTROL
ASSESSMENT OF BEAUVERIA-BASSIANA NOV EO-1 STRAIN, WHICH PRODUCES A RED PIGMENT FOR MICROBIAL CONTROL
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DOI：
10.1007/bf02921852
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发表时间：
1994-01-01
影响因子：
3
通讯作者：
LANDA, Z
中科院分区：
文献类型：
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作者：
EYAL, J;MABUD, MA;LANDA, Z
一种新的球孢白僵菌（Beauveria bassiana 11月o -1）菌株（ATCC 74037）在固体和液体培养中产生一种红色色素，已从感染的白蝇中分离出来。提取红色色素，质谱鉴定为卵孢素，一种强效的二苯醌类真菌毒素。为了评估这种虫生真菌在微生物控制方面的潜力，我们开发了一种菌丝珠制剂，作为致病分生孢子和卵孢子素的来源。菌丝珠制剂是一种稳定的真菌载体。分生孢子生产和萌发的研究已经揭示了菌丝体珠生存能力是100%在1年时期储存在4度c分生孢子的孢子产量从菌丝体珠子已经大幅下降/时间从一个初始值为1.5 x 10(8)孢子/珠3 x 10(5)孢子/珠一年后存储在4度c。然而,菌丝珠制定继续生产oosporein琼脂媒体,在同一强度在整个1年。通过对球孢白僵菌（Beauveria bassiana 11 . EO-1）与已知虫生真菌白僵菌（Beauveria sp.）和Paceilomyces sp.)的体外和体内小温室试验，发现球孢白僵菌（Beauveria bassiana 11 . EO-1）对叶害虫（如粉虱和粉虱）和土壤昆虫（如柑橘根象鼻虫）具有较高的致病性。利用球孢白僵菌（Beauveria bassiana 11 . EO-1）的菌丝球作为分生孢子和卵孢子素的来源，在防治各种害虫方面具有广阔的应用前景。
A new strain of the fungus Beauveria bassiana Nov. EO-1 (ATCC 74037), which produces a red pigment in solid and liquid culture, has been isolated from an infected whitefly. The red pigment was extracted and has been identified by mass spectrometry as oosporein, a potent dibenzoquinone mycotoxin.In order to assess the potential of this entomogenous fungi for microbial control purposes, a mycelium bead formulation was developed as a source for pathogenic conidial spores and oosporein production. The mycelium bead preparation was found to be a stable fungal carrier. Conidiation and germination studies have revealed the mycelium bead viability is 100% over a 1-yr period when stored at 4 degrees C. Conidial spore production from the mycelium beads has been falling substantially per time from an initial value of 1.5 x 10(8) spores per bead to 3 x 10(5) spores per bead after a year storage at 4 degrees C. However, the mycelium bead formulation continues to produce oosporein on agar media, at the same intensity throughout the 1 yr period.In in vitro and in vivo small scale greenhouse experiments Beauveria bassiana Nov. EO-1 were compared with known entomogenous fungi, Beauveria sp. and Paceilomyces sp. Beauveria bassiana Nov. EO-1 was found to have a high pathogenicity against foliage insect pests (e.g., whiteflies and mealy bugs), and against soil insects (e.g., citrus root weevils). The utilization of a mycelium bead based on this strain, Beauveria bassiana Nov. EO-1, as a source of conidial spores and oosporein may have broad applications for the control of various insect pests.