Heat shock protein 60 is a high-affinity high-density lipoprotein binding protein
Heat shock protein 60 is a high-affinity high-density lipoprotein binding protein
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DOI：
10.1006/bbrc.2000.3663
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发表时间：
2000-10-14
影响因子：
3.1
通讯作者：
Eggerman, TL
中科院分区：
文献类型：
--
作者：
Bocharov, AV;Vishnyakova, TG;Eggerman, TL
在人细胞系和原代培养肝细胞的质膜制剂中发现了一种新的55-kDa HDL/载脂蛋白结合蛋白，通过配体免疫印迹分析HDL、apoA-I和apoA-II与部分纯化的S5-kDa PA-I质膜制剂的特异性结合表明，K-d,K-HDL = 50 nM（10马克杯/毫升），K-d,K-apoA-II = 20 nM（0.4马克杯/毫升），K-d,K-apoA-I = 330 nM（10马克杯/毫升）。对分离自人pa - 1细胞的质膜制备物进行SDS-PAGF制备电泳后，收集具有apoA-II结合活性的组分，浓缩后进行二维电泳。55-kDa蛋白带内部微蛋白测序显示，结合蛋白为热休克蛋白60 (hsp60)， hsp60单克隆抗体LK-1阻断apoA-II与55-kDa HBP制剂的结合。总之，这些结果提供了一种潜在的机制来解释针对hsp60的免疫与动脉粥样硬化发展之间的已知关联，(C) 2000学术出版社。
A new 55-kDa HDL/apolipoprotein binding protein was demonstrated in plasma membrane preparations of the human cell lines and primary cultured hepatocytes, Analysis of specific binding by ligand immunoblots of HDL, apoA-I, and apoA-II to a partially purified S5-kDa PA-I plasma membrane preparation demonstrated a K-d,K-HDL = 50 nM (10 mug/ml), K-d,K-apoA-II = 20 nM (0.4 mug/ml), and K-d,K-apoA-I = 330 nM (10 mug/ml). Following preparative SDS-PAGF electrophoresis of a plasma membrane preparation isolated from human PA-I cells, fractions with apoA-II binding activity were collected, concentrated, and subjected to two-dimensional electrophoresis. Internal microprotein sequencing of the 55-kDa protein band revealed the binding protein as being heat shock protein 60 (hsp60), The hsp60 monoclonal antibody LK-1 blocked apoA-II binding to the 55-kDa HBP preparation. In summary, these results provide a potential mechanism to explain the known association between immunity developed against hsp60 and the development of atherosclerosis, (C) 2000 Academic Press.