Cross-talk between salicylic acid and NaCl-generated reactive oxygen species and nitric oxide in tomato during acclimation to high salinity
Cross-talk between salicylic acid and NaCl-generated reactive oxygen species and nitric oxide in tomato during acclimation to high salinity
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DOI：
10.1111/j.1399-3054.2011.01461.x
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发表时间：
2011-06-01
影响因子：
6.4
通讯作者：
Tari, Irma
中科院分区：
文献类型：
--
作者：
Gemes, Katalin;Poor, Peter;Tari, Irma
水杨酸（SA）产生的过氧化氢（H2 O2）和一氧化氮（NO）被认为是对各种应激源交叉耐受的功能环节。SA刺激的预适应状态有利于番茄（Solanum lycopersicum cv. Rio Fuego）。在整个植物水平上，SA诱导的大量H2 O2积累只有在高浓度（10-3-10-2 M），后来导致植物死亡。与暴露于100 mM NaCl的植物相比，过量积累的H2 O2与SA预处理后的盐胁迫反应无关。在根尖中，10-3-10-2 M SA引发活性氧（ROS）和NO的产生，伴随着细胞活力的下降。然而，SA的亚致死浓度，降低了盐胁迫对根尖ROS和NO产生的影响。高盐胁迫对植物氧化胁迫的减弱不仅发生在适应前，而且发生在细胞水平。当从控制叶片制备的原生质体暴露于SA在100 mM NaCl的存在下，NO和ROS的生产是低得多，细胞的活力高于盐处理的样品。这表明，SA和高盐度诱导的信号通路的串扰可能发生在ROS和NO的产生水平。脱落酸（阿坝），多胺和1-氨基环丙烷-1-羧酸，化合物积累在预处理的植物，提高二苯碘敏感的ROS和NO水平，但与其他相比，阿坝和腐胺保存的原生质体的活力。
Hydrogen peroxide (H2O2) and nitric oxide (NO) generated by salicylic acid (SA) are considered to be functional links of cross-tolerance to various stressors. SA-stimulated pre-adaptation state was beneficial in the acclimation to subsequent salt stress in tomato (Solanum lycopersicum cv. Rio Fuego). At the whole-plant level, SA-induced massive H2O2 accumulation only at high concentrations (10-3-10-2 M), which later caused the death of plants. The excess accumulation of H2O2 as compared with plants exposed to 100 mM NaCl was not associated with salt stress response after SA pre-treatments. In the root tips, 10-3-10-2 M SA triggered the production of reactive oxygen species (ROS) and NO with a concomitant decline in the cell viability. Sublethal concentrations of SA, however, decreased the effect of salt stress on ROS and NO production in the root apex. The attenuation of oxidative stress because of high salinity occurred not only in pre-adapted plants but also at cell level. When protoplasts prepared from control leaves were exposed to SA in the presence of 100 mM NaCl, the production of NO and ROS was much lower and the viability of the cells was higher than in salt-treated samples. This suggests that, the cross-talk of signalling pathways induced by SA and high salinity may occur at the level of ROS and NO production. Abscisic acid (ABA), polyamines and 1-aminocyclopropane-1-carboxylic acid, the compounds accumulating in pre-treated plants, enhanced the diphenylene iodonium-sensitive ROS and NO levels, but, in contrast to others, ABA and putrescine preserved the viability of protoplasts.