Brassinosteroids, microtubules and cell elongation in Arabidopsis thaliana.: I.: Molecular, cellular and physiological characterization of the Arabidopsis bul1 mutant, defective in the Δ7-sterol-C5-desaturation step leading to brassinosteroid biosynthesis
Brassinosteroids, microtubules and cell elongation in Arabidopsis thaliana.: I.: Molecular, cellular and physiological characterization of the Arabidopsis bul1 mutant, defective in the Δ7-sterol-C5-desaturation step leading to brassinosteroid biosynthesis
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DOI：
10.1007/s004250000466
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发表时间：
2001-04-01
期刊：
影响因子：
4.3
通讯作者：
Sangwan, RS
中科院分区：
文献类型：
--
作者：
Catterou, M;Dubois, F;Sangwan, RS
虽然细胞伸长是植物形态建成的基本功能，但许多参与这一过程的分子事件仍然是未知的。在这项工作中，一个非常矮小的突变体，最初命名为bul，被用来研究植物发育的主要过程之一，细胞伸长。遗传分析表明，BUL基因座与3号染色体上的nga 172标记连锁。最近，在定位拟南芥新的dwf 7突变后，它是与ste 1等位的，据报道dwf 7也与相同的标记连锁。bull-1突变体的甾醇分析表明，bull-1在导致油菜素甾醇生物合成的δ（7）-甾醇-C5-去饱和步骤中存在缺陷。考虑到这些发现，我们将我们的bul突变体命名为bul 1 -1/dwf 7 -3/ste 1 -4。bul 1 z-1突变体的特点是一个非常矮小的表型，发育延迟和生育能力降低。突变体叶片呈深绿色，这可能是由于气孔持续关闭造成的。bull-1突变体在黑暗中表现出部分去黄化表型。油菜素类固醇的细胞表征和拯救实验证明了BUL 1 -1蛋白参与油菜素类固醇依赖的植物生长过程。
Although cell elongation is a basic function of plant morphogenesis, many of the molecular events involved in this process are still unknown. Tn this work an extremely dwarf mutant, originally named bul, was used to study one of the main processes of plant development, cell elongation. Genetic analyses revealed that the BUL locus was linked to the nga 172 marker on chromosome 3. Recently, after mapping the new dwf7 mutation of Arabidopsis, which is allelic to ste1, it was reported that dwf7 is also linked to the same marker. Sterol analyses of the bull-1 mutant indicated that bull-1 is defective in the Delta (7)-sterol-C5-desaturation step leading to brassinosteroid biosynthesis. Considering these findings, we designated our bul mutant as bul1-1/dwf7-3/ste1-4. The bul1z-1 mutant was characterized by a very dwarf phenotype, with delayed development and reduced fertility. The mutant leaves had a dark-green colour, which was probably due to continuous stomatal closure. The bull-1 mutant showed a partially de-etiolated phenotype in the dark. Cellular characterization and rescue experiments with brassinosteroids demonstrated the involvement of the BUL1-1 protein in brassinosteroid-dependent plant growth processes.