Effect of moxibustion on the expression of GDNF and its receptor GFRα3 in the colon and spinal cord of rats with irritable bowel syndrome
Effect of moxibustion on the expression of GDNF and its receptor GFRα3 in the colon and spinal cord of rats with irritable bowel syndrome
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DOI：
10.1136/acupmed-2017-011455
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发表时间：
2019-08-01
影响因子：
2.5
通讯作者：
Wang, Xiaomei
中科院分区：
文献类型：
--
作者：
Qi, Qin;Wu, Huangan;Wang, Xiaomei
背景资料：已发现莫西沙星治疗可改善肠易激综合征（IBS）患者的临床症状，包括腹痛、腹泻和便秘。本研究旨在探讨艾灸治疗肠易激综合征的作用机制。方法：采用结肠扩张（CRD）刺激和芥子油灌肠建立大鼠IBS模型。将大鼠随机分为正常组、模型组、温和灸组、电针组、益生菌组和地西他滨组。在末次给药后90 min内测定腹部退缩反射（AWR）评分。采用免疫组化和实时荧光定量PCR方法分别检测结肠和脊髓GDNF/GFR α 3蛋白和mRNA的表达。结果：IBS模型组大鼠AWR评分明显高于正常组（P
Background: Moxibustion treatment has been found to ameliorate clinical symptoms including abdominal pain, diarrhoea and constipation in patients with irritable bowel syndrome (IBS). Herein we investigated the mechanisms underlying the use of moxibustion in a rat model of IBS. Methods: In our study, an IBS model was established in rats by colorectal distension (CRD) stimulus and mustard oil enema. The rats were randomly divided into a normal group, model group, mild moxibustion group, electroacupuncture group, probiotic group and dicetel group. Abdominal withdrawal reflex (AWR) scores were determined within 90 min of the last treatment. The expression of GDNF/GFR alpha 3 protein and mRNA in the colon and spinal cord were detected by immunohistochemistry and quantitative real-time-PCR, respectively. Results: The IBS model rats had significantly higher AWR scores than the normal group (P