Probing DNA Stiffness through Optical Fluctuation Analysis of Plasmon Rulers.
Probing DNA Stiffness through Optical Fluctuation Analysis of Plasmon Rulers.
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DOI：
10.1021/acs.nanolett.5b01725
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发表时间：
2015-08-12
期刊：
影响因子：
10.8
通讯作者：
Reinhard BM
中科院分区：
文献类型：
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作者：
Chen T;Hong Y;Reinhard BM
生物聚合物束缚的金或银纳米颗粒之间的依赖距离的等离子激元耦合形成了所谓的等离子体激元尺子的基础。虽然传统的等离子体激元标尺的应用侧重于检测远场光谱中的奇异事件，但在这封信中，我们对限于流体类脂膜的等离子体激元尺子的粒子间热分离变化引起的连续光谱波动进行了比率分析。我们用不同的脱氧核糖核酸链来表征等离激元尺子，并展示了检测和量化等离体尺电位和系留刚性差异的能力。分析了锚链的性质(单链DNA与双链DNA)和锚链长度的影响。通过光学涨落分析对单个等离子体激元尺子中粒子间间隔的连续变化进行表征，提供了有关位于深亚衍射极限粒子间隙范围内的系链分子(S)构象柔性的额外信息，并增强了等离子体激元尺子作为生物物理和纳米技术工具的多功能性。
The distance dependent plasmon coupling between biopolymer tethered gold or silver nanoparticles forms the foundation for the so-called Plasmon Rulers. While conventional Plasmon Ruler applications focus on the detection of singular events in the far-field spectrum, we perform in this Letter a ratiometric analysis of the continuous spectral fluctuations arising from thermal interparticle separation variations in Plasmon Rulers confined to fluid lipid membranes. We characterized Plasmon Rulers with different DNA tethers and demonstrate the ability to detect and quantify differences in the Plasmon Ruler potential and tether stiffness. The influence of the nature of the tether (single-stranded versus double-stranded DNA) and the length of the tether is analyzed. The characterization of the continuous variation of the interparticle separation in individual Plasmon Rulers through optical fluctuation analysis provides additional information about the conformational flexibility of the tether molecule(s) located in the confinement of the deeply subdiffraction limit interparticle gap and enhances the versatility of Plasmon Rulers as tool in Biophysics and Nanotechnology.