SIMULTANEOUS SINGLE UNIT RECORDING INVITRO WITH A PHOTOETCHED LASER DEINSULATED GOLD MULTI-MICRO-ELECTRODE SURFACE
SIMULTANEOUS SINGLE UNIT RECORDING INVITRO WITH A PHOTOETCHED LASER DEINSULATED GOLD MULTI-MICRO-ELECTRODE SURFACE
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DOI：
10.1109/tbme.1979.326402
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发表时间：
1979-01-01
影响因子：
4.6
通讯作者：
GROSS, GW
中科院分区：
文献类型：
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作者：
GROSS, GW
用宽12 μm、厚2 μm的光蚀刻金导体，在玻璃板上，用8 ns的紫外激光脉冲使其尖端去绝缘，记录了蜗牛脑神经节的单个细胞外活动。一个由36个这样的导体组成的固定阵列，在0.5 mm x 1 mm的区域内终止于六行六列，能够同时监测来自许多神经元的单个单元活动。通常从这些神经节的主要40 μm直径细胞中观察到300-500 μV的尖峰振幅。巨神经元通常产生超过3 mV的信号幅度，这些信号幅度可以被许多电极同时看到。只需将局部去鞘的神经节在其自身重量下放在记录区域的一个浅的林格池中即可监测信号。3-4 μm厚绝缘层中直径为10 μm的凹坑在1 kHz时的阻抗为2-4 MΩ。该金属电解质界面的电容约为0.5 pF/μm2，表明UV激光产生部分胶体金表面。通过这种凹进的尖端设计，如果电极阻抗低于4 MΩ，分流阻抗高于30 MΩ，并且不允许胶质细胞重新绝缘金导体的末端，则可以确保来自小神经元的同时单个单元记录。
Flat photoetched gold conductors 12 μm wide and 2 μm thick, situated on a glass plate and deinsulated at their tips with single 8 ns UV laser pulses, have been utilized to record single unit extra-cellular activity from brain ganglia of the snail Helix pomatia. A fixed array of 36 such conductors, terminating in six rows and six columns in a 0.5 mm x 1 mm area, is capable of monitoring simultaneous single unit activity from numerous neurons. Spike amplitudes of 300-500 μV are generally observed from the predominant 40 μm diameter cells of these ganglia. Giant neurons usually produce signal amplitudes of over 3 mV that are simultaneously seen by many electrodes. Signals can be monitored by merely resting a locally desheathed ganglion on the recording area under its own weight in a shallow pool of Ringer. A 10 μm diameter crater in a 3-4 μm thick insulation layer has an impedance of 2-4 MΩat 1 kHz. The capacitance of this metal electrolyte interface is about 0.5 pF/μm2, suggesting that the UV laser produces a partially colloidal gold surface. With this recessed tip design, simultaneous single unit recording from small neurons appears ensured if electrode impedances are below 4 MΩ, shunt impedances are above 30 MΩ, and glia cells are not allowed to reinsulate the end of the gold conductor.